Möröy Tarik, Heyd Florian
Institut de Recherches Cliniques de Montréal, IRCM, Montréal, QC, Canada.
RNA. 2007 Aug;13(8):1155-71. doi: 10.1261/rna.554607. Epub 2007 Jun 11.
Alternative splicing is widely believed to have a major impact on almost all biological processes since it increases proteome complexity and thereby controls protein function. Recently, gene targeting in mice has been used to create in vivo models to study the regulation and consequences of alternative splicing. The evidence accumulated so far argues for a nonredundant, highly specific role of individual splicing factors in mammalian development, and furthermore, demonstrates the importance of distinct protein isoforms in vivo. In this review, we will compare phenotypes of mouse models for alternative splicing to crystallize common themes and to put them into perspective with the available in vitro data.
人们普遍认为，可变剪接对几乎所有生物过程都有重大影响，因为它增加了蛋白质组的复杂性，从而控制蛋白质功能。最近，小鼠基因靶向技术已被用于创建体内模型，以研究可变剪接的调控及其后果。迄今为止积累的证据表明，单个剪接因子在哺乳动物发育中具有非冗余、高度特异性的作用，此外，还证明了不同蛋白质异构体在体内的重要性。在这篇综述中，我们将比较可变剪接小鼠模型的表型，以梳理出共同主题，并结合现有的体外数据对其进行解读。
