Satoh S, Suzuki Y, Harada T, Ikegaki I, Asano T, Shibuya M, Sugita K, Saito A
Department of Neurosurgery, Nagoya University School of Medicine, Japan.
Brain Res Bull. 1991 Nov;27(5):663-8. doi: 10.1016/0361-9230(91)90042-i.
A possible role of platelet-derived 5-HT was examined concerning the pathogenesis of cerebral vasospasm. Intracisternal injection of 5 ml of platelet-rich plasma (PRP; approximately 7.5 x 10(8) platelets) induced not only acute (1 h) but also chronic (7 days) angiographically evident cerebral vasospasm in dogs. Sympathetic perivascular nerves on cerebral arteries showed no remarkable change following repeated injections of PRP, as the dense distribution of catecholamine-fluorescence and neuropeptide Y-like immunoreactive nerve fibers on Day 7 was comparable to findings in the preinjection controls. While there were no 5-HT-immunoreactive fibers in the cerebral arteries of control animals, numerous 5-HT-immunoreactive fibers were present in the PRP-injected animals. These results suggest that 5-HT, presumably released from extravasated platelets, may be taken up by nerve endings and act as a vasoconstrictor transmitter in the pathogenesis of chronic vasospasm.
研究了血小板衍生的5-羟色胺（5-HT）在脑血管痉挛发病机制中的可能作用。向犬脑池内注射5ml富含血小板的血浆（PRP；约7.5×10⁸个血小板）不仅可诱发急性（1小时）血管造影可见的脑血管痉挛，还可诱发慢性（7天）血管造影可见的脑血管痉挛。反复注射PRP后，脑动脉周围的交感神经未见明显变化，因为第7天儿茶酚胺荧光和神经肽Y样免疫反应性神经纤维的密集分布与注射前对照组的结果相当。虽然对照动物的脑动脉中没有5-HT免疫反应性纤维，但在注射PRP的动物中存在大量5-HT免疫反应性纤维。这些结果表明，推测从渗出的血小板释放的5-HT可能被神经末梢摄取，并在慢性血管痉挛的发病机制中作为血管收缩递质起作用。
