从秘鲁受畸形花叶病影响的利马豆植株中鉴定出的菜豆普通花叶病毒（BCMV）和菜豆普通坏死花叶病毒（BCMNV）毒株。

Strains of BCMV and BCMNV characterized from lima bean plants affected by deforming mosaic disease in Peru.

作者信息

Melgarejo T A, Lehtonen M T, Fribourg C E, Rännäli M, Valkonen J P T

机构信息

Departamento de Fitopatologia, Universidad Nacional Agraria La Molina (UNALM), Lima, Peru.

出版信息

Arch Virol. 2007;152(10):1941-9. doi: 10.1007/s00705-007-1008-z. Epub 2007 Jun 14.

DOI:10.1007/s00705-007-1008-z

PMID:17564759

Abstract

Viruses of the species Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) were simultaneously detected by the different size of PCR amplicons in lima bean plants (Phaseolus lunatus) displaying deforming mosaic symptoms in Peru. Phylogenetic analysis of partial deduced CP amino acid sequences indicated that the Peruvian BCMV isolates belong to new strains. One isolate differed from the other Peruvian isolates, and also from the ten previously described BCMV strains recognized by responses on differential bean varieties. The sequence of the 3'-proximal part (2547 nucleotides) of the genome confirmed that this isolate also belongs to BCMV.

摘要

在秘鲁表现出变形花叶症状的利马豆植株（菜豆）中，通过不同大小的PCR扩增子同时检测到了菜豆普通花叶病毒（BCMV）和菜豆普通花叶坏死病毒（BCMNV）这两个种的病毒。对部分推导的CP氨基酸序列进行系统发育分析表明，秘鲁的BCMV分离株属于新的株系。其中一个分离株与其他秘鲁分离株不同，也与之前通过在鉴别菜豆品种上的反应所确认的10个BCMV株系不同。基因组3'-近端部分（2547个核苷酸）的序列证实该分离株也属于BCMV。