沼泽红假单胞菌CGA009铁氧化还原蛋白还原酶的结晶及初步X射线衍射研究。
Crystallization and preliminary X-ray diffraction studies of a ferredoxin reductase from Rhodopseudomonas palustris CGA009.
作者信息
Peng Ying, Xu Feng, Bell Stephen G, Wong Luet-Lok, Rao Zihe
机构信息
Laboratory of Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):422-5. doi: 10.1107/S1744309107017411. Epub 2007 Apr 20.
Abstract
Palustrisredoxin reductase from Rhodopseudomonas palustris CGA009, a member of the oxygenase-coupled NADH-dependent ferredoxin reductase (ONFR) family, catalyzes electron transfer from NADH to ferredoxins. It is an essential component of the cytochrome P450 systems in R. palustris CGA009, a model organism with diverse metabolic pathways. Here, the crystallization of palustrisredoxin reductase is reported. The crystals belong to the trigonal space group P3(2)21, with unit-cell parameters a = 107.5, b = 107.5, c = 69.9 A, and diffract to 2.2 A resolution on a synchrotron source.
摘要
来自沼泽红假单胞菌CGA009的沼泽红氧还蛋白还原酶是加氧酶偶联的NADH依赖性铁氧还蛋白还原酶（ONFR）家族的成员，催化电子从NADH转移至铁氧还蛋白。它是具有多种代谢途径的模式生物——沼泽红假单胞菌CGA009中细胞色素P450系统的重要组成部分。在此，报道了沼泽红氧还蛋白还原酶的晶体结构。晶体属于三方晶系空间群P3(2)21，晶胞参数a = 107.5、b = 107.5、c = 69.9 Å，在同步辐射光源上衍射至2.2 Å分辨率。
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