Tasevska N, Runswick S A, McTaggart A, Bingham S A
MRC Dunn Human Nutrition Unit, University of Cambridge, Cambridge, UK.
Eur J Clin Nutr. 2008 Sep;62(9):1139-47. doi: 10.1038/sj.ejcn.1602829. Epub 2007 Jun 13.
To investigate 24-h urinary thiamine as a potential biomarker for thiamine intake for use in validation studies to assess the validity of dietary intake data collected by self-reporting dietary methods.
Seven male and six female healthy participants living for 30 days in a metabolic suite under strictly controlled conditions consuming their usual diet as assessed beforehand from four consecutive 7-day food diaries kept at home. During the 30-day study, all 24-h urine specimens were collected, validated for their completeness and analysed for thiamine.
Thirty-day mean (+/-s.d.) calculated thiamine intake was 2.22+/-0.55 mg/day. Thirty-day mean (+/-s.d.) urinary excretion of thiamine was 526.5+/-193.0 microg/day (24.7+/-8.10% of intake). There was a highly significant correlation between individuals' 30-day means of thiamine intake and their mean excretion level (r=0.720; P=0.006), where 1 mg of thiamine intake predicted 268.2 microg of thiamine in urine. The correlations between intake and excretion remained significant when measurement from a single 24-h urine collection was used (r=0.56).
Twenty-four-hour urinary thiamine can be used as a concentration biomarker for thiamine intake in dietary validation studies.
研究24小时尿硫胺素作为硫胺素摄入量潜在生物标志物的情况，用于验证研究，以评估通过自我报告饮食方法收集的饮食摄入数据的有效性。
7名男性和6名女性健康参与者，在代谢套房中严格控制条件下生活30天，食用他们平常的饮食，平常饮食根据之前在家中连续记录的4份7天食物日记进行评估。在30天的研究期间，收集了所有24小时尿液样本，验证其完整性并分析硫胺素。
30天计算的硫胺素平均摄入量（±标准差）为2.22±0.55毫克/天。30天硫胺素的平均尿排泄量（±标准差）为526.5±193.0微克/天（摄入量的24.7±8.10%）。个体30天硫胺素摄入量均值与其平均排泄水平之间存在高度显著相关性（r=0.720；P=0.006），其中1毫克硫胺素摄入量可预测尿中268.2微克硫胺素。当使用单次24小时尿液收集测量时，摄入量与排泄量之间的相关性仍然显著（r=0.56）。
在饮食验证研究中，24小时尿硫胺素可作为硫胺素摄入量的浓度生物标志物。
