Da Costa Grégory, Mouret Liza, Chevance Soizic, Le Rumeur Elisabeth, Bondon Arnaud
RMN-Interactions Lipides Protéines, UMR CNRS 6026, IFR 140, PRISM, Université de Rennes 1, 35043, Rennes Cedex, France.
Eur Biophys J. 2007 Nov;36(8):933-42. doi: 10.1007/s00249-007-0186-7. Epub 2007 Jun 13.
Detailed characterization of protein, peptide or drug interactions with natural membrane is still a challenge. This review focuses on the use of nuclear magnetic resonance (NMR) for the analysis of interaction of molecules with small unilamellar vesicles (SUV). These phospholipid vesicles are often used as model membranes for fluorescence or circular dichroism experiments. The various NMR approaches for studying molecule-lipid association are reviewed. After a brief survey of the SUV characterization, the use of heteronuclear NMR (phosphorous, carbon, fluorine) is described. Applications of proton NMR through transferred nuclear Overhauser effect to perform structural determination of peptide are presented. Special care is finally given to the influence of the kinetic of the interactions for the proton NMR of bound molecules in SUV, which can constitute a good model for the study of dynamical processes at the membrane surface.
蛋白质、肽或药物与天然膜相互作用的详细表征仍然是一项挑战。本综述重点关注利用核磁共振（NMR）分析分子与小单层囊泡（SUV）的相互作用。这些磷脂囊泡常被用作荧光或圆二色性实验的模型膜。本文综述了研究分子 - 脂质缔合的各种NMR方法。在简要概述SUV表征之后，描述了异核NMR（磷、碳、氟）的应用。介绍了通过转移核Overhauser效应利用质子NMR进行肽结构测定的应用。最后特别关注了SUV中结合分子的质子NMR相互作用动力学的影响，SUV可作为研究膜表面动态过程的良好模型。
