Yu Ju-Kyung, Graznak Elizabeth, Breseghello Flavio, Tefera Hailu, Sorrells Mark E
Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, USA.
BMC Plant Biol. 2007 Jun 12;7:30. doi: 10.1186/1471-2229-7-30.
Tef [Eragrostis tef (Zucc.) Trotter] is the major cereal crop in Ethiopia. Tef is an allotetraploid with a base chromosome number of 10 (2n = 4x = 40) and a genome size of 730 Mbp. The goal of this study was to identify agronomically important quantitative trait loci (QTL) using recombinant inbred lines (RIL) derived from an inter-specific cross between E. tef and E. pilosa (30-5).
Twenty-two yield-related and morphological traits were assessed across eight different locations in Ethiopia during the growing seasons of 1999 and 2000. Using composite interval mapping and a linkage map incorporating 192 loci, 99 QTLs were identified on 15 of the 21 linkage groups for 19 traits. Twelve QTLs on nine linkage groups were identified for grain yield. Clusters of more than five QTLs for various traits were identified on seven linkage groups. The largest cluster (10 QTLs) was identified on linkage group 8; eight of these QTLs were for yield or yield components, suggesting linkage or pleotrophic effects of loci. There were 15 two-way interactions of loci to detect potential epistasis identified and 75% of the interactions were derived from yield and shoot biomass. Thirty-one percent of the QTLs were observed in multiple environments; two yield QTLs were consistent across all agro-ecology zones. For 29.3% of the QTLs, the alleles from E. pilosa (30-5) had a beneficial effect.
The extensive QTL data generated for tef in this study will provide a basis for initiating molecular breeding to improve agronomic traits in this staple food crop for the people of Ethiopia.
画眉草（Eragrostis tef (Zucc.) Trotter）是埃塞俄比亚的主要谷类作物。画眉草是异源四倍体，基本染色体数为10（2n = 4x = 40），基因组大小为730兆碱基对。本研究的目的是利用从画眉草和毛花画眉草（30 - 5）种间杂交获得的重组自交系（RIL）来鉴定具有重要农艺性状的数量性状位点（QTL）。
在1999年和2000年生长季节，在埃塞俄比亚的八个不同地点对22个与产量相关和形态性状进行了评估。利用复合区间作图法和包含192个位点的连锁图谱，在21个连锁群中的15个上鉴定出了与19个性状相关的99个QTL。在9个连锁群上鉴定出了与籽粒产量相关的12个QTL。在7个连锁群上鉴定出了各种性状的5个以上QTL的簇。最大的簇（10个QTL）在连锁群8上被鉴定出来；其中8个QTL与产量或产量构成因素有关，表明这些位点存在连锁或多效性效应。鉴定出了15个位点的双向互作以检测潜在的上位性，其中75%的互作来自产量和地上部生物量。31%的QTL在多个环境中被观察到；两个产量QTL在所有农业生态区都是一致的。对于29.3%的QTL，来自毛花画眉草（30 - 5）的等位基因具有有益效应。
本研究中为画眉草生成的大量QTL数据将为启动分子育种以改善这种埃塞俄比亚主食作物的农艺性状提供基础。
