Hu Wei-Hong, Qiao Jie, Li Mei-Zhi
Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100083, China.
Zhonghua Yi Xue Za Zhi. 2007 Mar 20;87(11):721-4.
To investigate the association of monocyte chemoattractant protein-1 (MCP-1) and the clinical characteristics of polycystic ovary syndrome: (PCOS).
Fasting peripheral venous blood samples were collected on the 2nd or 3rd day of the menstrual cycle or when there was no dominant follicle shown by ultrasonography after amenorrhea from 65 POCS patients, aged 30 +/- 3, 27 being attributed to the obese group according the body mass index (BMI) and 38 to the non-obese group, and 40 patients with infertility, aged 31 +/- 3, as controls, subdivided into obese and non-obese subgroups (both n = 20), and then the samples of serum. Were obtained. The level of MCP-1 was examined by ELISA. The levels of prolactin, luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol, and testosterone (T) were assayed with chemoluminescence methods, and the level of androstenedione was detected by radioimmunoassay. The level of serum insulin was detected by chemoluminescence method and the serum glucose (SG) was detected by oxidase enzymic method. Insulin sensitivity index (ISI) and homeostasis model assessment insulin resistance (HOMA-IR) were calculated.
(1) The levels of serum LH, T, and LH/FSH of the obese and non-obese POCS subgroups were all significantly higher than the corresponding obese and non-obese control subgroups (all P < 0.05). (2) The level of MCP-1 of the non-obese PCOS subgroup was (98 +/- 67) ng/ml, significantly higher than that of the non-obese controls [(58 +/- 41 mg/L, P < 0.05)]. (3) Pearson correlation showed that serum PCP-1 was significantly positively correlated with BMI (r = 0.339, P = 0.000). LH (r = 0.224, P = 0.024)), and HOMA-IR (r = 0.239, P = 0.016), and significantly negatively correlated with ISI (r = -0.250, P = 0.0006). (3) Multiple regression analysis showed that BMI and LH were the principal factors influencing the level of MCP-1 in the POCS patients.
The serum level of MCP-1 is associated with the LH level in POCS patients. POCS may a chronic inflammatory disease. MCP-1 is likely to participate in obesity, hyperinsulinemia, and insulin resistance in POCS.
探讨单核细胞趋化蛋白-1（MCP-1）与多囊卵巢综合征（PCOS）临床特征的相关性。
收集65例年龄30±3岁的PCOS患者在月经周期第2或3天或闭经后超声检查未发现优势卵泡时的空腹外周静脉血样本，根据体重指数（BMI）将27例归为肥胖组，38例归为非肥胖组，另选40例年龄31±3岁的不孕患者作为对照，也分为肥胖和非肥胖亚组（每组n = 20），然后获取血清样本。采用酶联免疫吸附测定法（ELISA）检测MCP-1水平。采用化学发光法检测催乳素、黄体生成素（LH）、卵泡刺激素（FSH）、雌二醇和睾酮（T）水平，采用放射免疫测定法检测雄烯二酮水平。采用化学发光法检测血清胰岛素水平，采用氧化酶法检测血清葡萄糖（SG）。计算胰岛素敏感性指数（ISI）和稳态模型评估胰岛素抵抗（HOMA-IR）。
（1）肥胖和非肥胖PCOS亚组的血清LH、T及LH/FSH水平均显著高于相应的肥胖和非肥胖对照亚组（均P < 0.05）。（2）非肥胖PCOS亚组的MCP-1水平为（98±67）ng/ml，显著高于非肥胖对照组[（58±41）mg/L，P < 0.05]。（3）Pearson相关性分析显示，血清PCP-1与BMI（r = 0.339，P = 0.000）、LH（r = 0.224，P = 0.024）及HOMA-IR（r = 0.239，P = 0.016）显著正相关，与ISI（r = -0.250，P = 0.0006）显著负相关。（3）多元回归分析显示，BMI和LH是影响PCOS患者MCP-1水平的主要因素。
PCOS患者血清MCP-1水平与LH水平相关。PCOS可能是一种慢性炎症性疾病。MCP-1可能参与PCOS患者的肥胖、高胰岛素血症及胰岛素抵抗过程。
