[GLI1在胰腺癌中的mRNA表达及其临床意义]
[mRNA expression of GLI1 in pancreatic carcinoma and clinical significance thereof].
作者信息
Guo Jie-fang, Li Zhao-shen, Jin Zhen-dong, Gao Jun, Gong Yan-fang, Jin Jing, Man Xiao-hua
机构信息
Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2007 Mar 27;87(12):826-8.
OBJECTIVE
To investigate the mRNA expression of GLI1, a transcription regulator of Hedgehog signaling pathway, in human pancreatic carcinoma and to explore its clinical significance.
METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of GLI1 in the tumor tissues, tissues near tumor, and normal tissues obtained during operation from 25 pancreatic carcinoma patients.
RESULTS
The GLI1 mRNA expression rate of the tumor tissues was 68.0% (17/25), significantly higher than those of the tissues near tumor, and normal tissues [24.0% (6/25) and 0 (0/25) respectively, both P < 0.01]. The GLI1 mRNA expression rate was significantly associated with the differentiation degree of tumor tissue (P = 0.014), and not significantly associated with the tumor size, invasion, and metastasis (all P > 0.05).
CONCLUSION
GLI1 mRNA expression is strong in pancreatic carcinoma tissues and is significantly associated with the differentiation degree of tumor tissue. With diagnostic implication, GLI1 mRNA expression may be regarded as a parameter of determining the degree of malignancy and prognosis of pancreatic carcinoma.
目的
研究Hedgehog信号通路转录调节因子GLI1在人胰腺癌中的mRNA表达情况，并探讨其临床意义。
方法
采用逆转录聚合酶链反应（RT-PCR）检测25例胰腺癌患者手术中获取的肿瘤组织、肿瘤旁组织及正常组织中GLI1的mRNA表达。
结果
肿瘤组织中GLI1 mRNA表达率为68.0%（17/25），显著高于肿瘤旁组织和正常组织[分别为24.0%（6/25）和0（0/25），P均<0.01]。GLI1 mRNA表达率与肿瘤组织分化程度显著相关（P = 0.014），与肿瘤大小、侵袭及转移无显著相关性（P均>0.05）。
结论
胰腺癌组织中GLI1 mRNA表达较强，且与肿瘤组织分化程度显著相关。具有诊断意义，GLI1 mRNA表达可作为判断胰腺癌恶性程度及预后的一个指标。
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