Wang Xia, Yang Yu-jia, Jia Yan-jie, Yu Xiao-he, Zhong Le, Xie Min, Wang Xiao-li
Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhonghua Yi Xue Za Zhi. 2007 Mar 27;87(12):847-50.
To investigate the best site of transplantation of neural stem cells (NSCs) into the brain to treat hypoxic-ischemic damage (HIBD).
Forty-eight 7-day-old Spraque-Dawley rats underwent ligation of the left common carotid artery and exposure to 8% oxygen at 37 degrees C for 2 hours to establish HIBD models and then were randomly divided into 4 equal groups 3 days later: HIBD control group, HIBD + cortex transplantation group (CT group) undergoing NSC transplantation into the sensorimotor cortex, HIBD + hippocampus transplantation group (HT group) undergoing NSC transplantation into the hippocampus, and HIBD + ventricle transplantation group (VT group), undergoing NSC transplantation into the lateral ventricle. Since the rats were 40-day-old, they underwent radial maze water-seeking test and 4 sensorimotor tests. Then the brains of the rats were taken out to undergo histological examination by Nissl staining and immunohistochemistry to observer the 5-bromodeoxyuridine (BrdU) expression.
The times the rats took to find the water in all 3 arms of the maze were arranged form short to long in the order HT group < VT group < CT group < HIBD group with significant differences between the HT, VT, and CT groups and HIBD group (All P < 0.05) and between the groups HT and VT and the group CT (both P < 0.05). The results of the 4 sensorimotor tests were arranged from the best to the worst in the order CT group > VT group > HT group > HIBD group with significant differences between the groups CT and VT and the group HIBD (both P < 0.05). Nissl staining showed that the number of normal neurons in the cortex area from more to less were arranged in the order CT group > VT group > HT group > HIBD group; and the number of normal neurons in the CAI area from more to less were arranged in the order HT group > VT group > CT group > HIBD group.
Transplantation of NSC attenuates the brain damage and the cognitive and sensorimotor dysfunction after HIBD. Transplantation into the lateral ventricle is the most effective.
探讨神经干细胞（NSCs）脑内移植治疗缺氧缺血性脑损伤（HIBD）的最佳移植部位。
48只7日龄Spraque-Dawley大鼠行左侧颈总动脉结扎，并于37℃、8%氧气环境中暴露2小时以建立HIBD模型，3天后随机分为4组，每组12只：HIBD对照组、HIBD + 皮质移植组（CT组），将神经干细胞移植至感觉运动皮质；HIBD + 海马移植组（HT组），将神经干细胞移植至海马；HIBD + 脑室移植组（VT组），将神经干细胞移植至侧脑室。大鼠40日龄时，进行放射状迷宫寻水试验及4项感觉运动测试。然后取出大鼠脑行尼氏染色及免疫组化组织学检查，观察5-溴脱氧尿嘧啶核苷（BrdU）表达情况。
在迷宫所有3个臂中大鼠找到水所需时间由短到长依次为HT组＜VT组＜CT组＜HIBD组，HT组、VT组、CT组与HIBD组之间差异有统计学意义（均P＜0.05），HT组与VT组及CT组之间差异有统计学意义（均P＜0.05）。4项感觉运动测试结果由好到差依次为CT组＞VT组＞HT组＞HIBD组，CT组与VT组及HIBD组之间差异有统计学意义（均P＜0.05）。尼氏染色显示，皮质区正常神经元数量由多到少依次为CT组＞VT组＞HT组＞HIBD组；CA1区正常神经元数量由多到少依次为HT组＞VT组＞CT组＞HIBD组。
神经干细胞移植可减轻HIBD后脑损伤及认知和感觉运动功能障碍。移植至侧脑室最为有效。
