Ugurbas Suat Hayri, Can Murat, Koksal Murat, Dincel Aysun, Bozkurt Atilla, Bayar Banu
Department of Ophthalmology, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey.
Adv Ther. 2007 Mar-Apr;24(2):394-401. doi: 10.1007/BF02849909.
Intraocular levels of ofloxacin are documented after topical and systemic administration, but systemic administration of ofloxacin in ocular compression has not yet been studied. This study was undertaken to determine the intraocular penetration of systemic ofloxacin into aqueous and vitreous humor after the application of ocular compression in rabbit eyes. Ocular compression with the Honan balloon was applied for 30 min to the right eyes of 11 albino New Zealand white rabbits. After the application of ocular compression, 2 mg/mL of ofloxacin was administered intravenously. Samples from aqueous and vitreous humor were collected 30 min after infusion. Ofloxacin concentrations were determined through high-performance liquid chromatography. The mean aqueous level of ofloxacin was significantly higher in the compression group (2.40+/-1.00 microg/mL) than in the no-compression group (1.61+/-1.06 microg/mL) (P<.05). The mean vitreous concentrations of ofloxacin were 0.70+/-0.33 microg/mL and 0.50+/-0.18 microg/mL in the compression and no-compression groups, respectively. A significant difference was observed between vitreous levels of ofloxacin in the compression and no compression groups (P<.05). Ocular compression enhanced the penetration of ofloxacin in both aqueous and vitreous humor. The drug level in the aqueous humor was sufficient for the minimum inhibitory concentration for 90% of isolates (MIC90) to inhibit most microorganisms. Although the mean vitreous ofloxacin concentration was increased by previous ocular compression, it was not sufficiently above the MIC90 for most ocular pathogens that caused endophthalmitis.
局部和全身给药后均有关于眼内氧氟沙星水平的记录，但尚未研究在眼部加压情况下全身应用氧氟沙星的情况。本研究旨在确定在兔眼施加眼部加压后全身应用的氧氟沙星在房水和玻璃体液中的眼内渗透情况。使用河南球囊对11只白化新西兰白兔的右眼施加眼部加压30分钟。施加眼部加压后，静脉注射2mg/mL的氧氟沙星。输液30分钟后收集房水和玻璃体液样本。通过高效液相色谱法测定氧氟沙星浓度。加压组的氧氟沙星平均房水水平（2.40±1.00μg/mL）显著高于未加压组（1.61±1.06μg/mL）（P<0.05）。加压组和未加压组的氧氟沙星平均玻璃体液浓度分别为0.70±0.33μg/mL和0.50±0.18μg/mL。加压组和未加压组的玻璃体液中氧氟沙星水平存在显著差异（P<0.05）。眼部加压增强了氧氟沙星在房水和玻璃体液中的渗透。房水中的药物水平足以达到90%分离株的最低抑菌浓度（MIC90）以抑制大多数微生物。虽然先前的眼部加压使玻璃体液中的氧氟沙星平均浓度有所增加，但对于大多数引起眼内炎的眼部病原体来说，其浓度仍未充分高于MIC90。
