Simeonova P P, Kehayov I R, Kyurkchiev S D
Department of Molecular Immunology, Bulgarian Academy of Sciences, Sofia.
Clin Chim Acta. 1991 Sep 30;201(3):207-21. doi: 10.1016/0009-8981(91)90372-j.
An ELISA assay with monoclonal antibody (Mab 2F4) raised against human ventricular myosin heavy chains was developed and used to investigate human sera after myocardial infarction. The monoclonal antibody 2F4 was selected for its high affinity to soluble fragments of myosin heavy chains (subfragment-1) and for its appropriate tissue specificity. By including Mab 2F4 in a simple and rapid dot immunobinding assay sera from patients with acute chest pain and of persons without a history of heart disease were tested. Myosin was detected only in the sera of the patients with myocardial necrosis, confirmed by electrocardiographic data. Negative reactions in all control cases were found. The serum myosin fragments reactive with Mab 2F4 were characterized by immunoblot experiments and protein bands in the region about 43 kDa were found. It was concluded that the myocardial infarction can be demonstrated by detection of cardiac myosin heavy chain fragments in the patients' sera.
开发了一种使用针对人心室肌球蛋白重链产生的单克隆抗体（Mab 2F4）的酶联免疫吸附测定（ELISA），并用于研究心肌梗死后的人血清。选择单克隆抗体2F4是因为它对肌球蛋白重链的可溶性片段（亚片段-1）具有高亲和力，并且具有适当的组织特异性。通过将Mab 2F4纳入简单快速的斑点免疫结合测定中，对急性胸痛患者和无心脏病史者的血清进行了检测。仅在心肌坏死患者的血清中检测到肌球蛋白，心电图数据证实了这一点。在所有对照病例中均发现阴性反应。通过免疫印迹实验对与Mab 2F4反应的血清肌球蛋白片段进行了表征，并在约43 kDa区域发现了蛋白条带。得出的结论是，通过检测患者血清中的心肌肌球蛋白重链片段可以证明心肌梗死。
