Parra H J, Martin F, Monard F, Ngangoué N, Copin N, Bard J M, Qafli M, Vu Dac N, Duriez P, Fruchart J C
Service de Recherche sur les Lipoprotéines et l' Athérosclérose (SERLIA), Institut Pasteur, Lille, France.
Clin Genet. 1991 Oct;40(4):263-70. doi: 10.1111/j.1399-0004.1991.tb03094.x.
The immunoreactivity of apolipoprotein B (apo B) in plasma obtained from 238 unrelated black African male subjects from the People's Republic of Congo was analysed by non-competitive Enzyme Linked-Immunosorbent Assay (ELISA) with monoclonal BIP 45 anti-LDL antibody. The polymorphism detected by BIP 45 monoclonal antibody is identical to the Ag(c,g) polymorphism. Antibody BIP 45 distinguishes three apo B allotypes (immunophenotypes) encoded by the two allelic genes apo B Ag(c) and apo B Ag(g). Because of co-dominant transmission, genotypes may be inferred from allotypes, and it has been shown that BIP 45 binds strongly to the Ag(c) factor and only weakly to the allelic Ag(g) factor. Analysis of the Congolese plasma samples indicated that 67.65% of them bound BIP 45 with low affinity (Ag(c-,g+) genotype), 28.15% with intermediate affinity (Ag(c+,g+) genotype) and 4.20% with high affinity (Ag(c+,g-) genotype). According to the Hardy-Weinberg equilibrium, this corresponds to gene frequencies of 0.817 and 0.183 for the type Ag(g)/Ag(c) alleles, respectively. After adjustment for age and body-mass index, it was found that the Ag(c) allele decreases the apo B level by 9.62 mg/dl and that the Ag(g) allele increases apo B by 0.43 mg/dl. Therefore, as much as 4.30% of the genetic variance for apo B level could be accounted for by the Ag(c,g) gene locus.
采用非竞争性酶联免疫吸附测定法（ELISA），使用单克隆BIP 45抗低密度脂蛋白（LDL）抗体，对来自刚果共和国的238名无亲缘关系的非洲黑人男性受试者血浆中的载脂蛋白B（apo B）免疫反应性进行了分析。BIP 45单克隆抗体检测到的多态性与Ag(c,g)多态性相同。抗体BIP 45可区分由两个等位基因apo B Ag(c)和apo B Ag(g)编码的三种apo B同种异型（免疫表型）。由于共显性遗传，可以从同种异型推断基因型，并且已经表明BIP 45与Ag(c)因子强烈结合，而与等位基因Ag(g)因子仅微弱结合。对刚果血浆样本的分析表明，其中67.65%以低亲和力结合BIP 45（Ag(c-,g+)基因型），28.15%以中等亲和力结合（Ag(c+,g+)基因型），4.20%以高亲和力结合（Ag(c+,g-)基因型）。根据哈迪-温伯格平衡，这分别对应于Ag(g)/Ag(c)等位基因类型的基因频率为0.817和0.183。在对年龄和体重指数进行校正后，发现Ag(c)等位基因使apo B水平降低9.62 mg/dl，而Ag(g)等位基因使apo B水平升高0.43 mg/dl。因此，Ag(c,g)基因座可解释apo B水平遗传变异的4.30%。
