Ergen Neslihan Z, Dinler Gizem, Shearman Robert C, Budak Hikmet
Faculty of Engineering and Natural Sciences, Biological Sciences and Bioengineering Program, Sabanci University, Orhanli, Tuzla 34956 Istanbul, Turkey.
Gene. 2007 May 15;393(1-2):145-52. doi: 10.1016/j.gene.2007.02.005.
Differentially expressed genes in response to rust infection (Puccinia sp.) in creeping red fescue (Festuca rubra var. rubra) were identified and quantified using the mRNA differential display technique. The differentially induced genes were identified as homologs of mitogen-activated protein kinase (MAPK) 3 of Arabidopsis thaliana, stem rust resistance protein Rpg1 of barley and Hsp70 of Spinacia oleracea. The change in the steady state expression levels of these genes in response to rust infection was tested by Northern blot analysis and further quantified by real-time PCR. A steady accumulation of transcripts in the course of rust infection was observed. Full-length transcript of a fescue MPK-3 was obtained by RACE PCR. Its corresponding cDNA encodes a protein with a predicted MW of 42.5 kDa which was mapped onto the structural model of homologs MAPK to illustrate the corresponding MAPK signature motifs. This study, for the first time, presents evidence on the rust infection dependent metabolic pathways in creeping red fescue.
利用mRNA差异显示技术，鉴定并定量了匍匐紫羊茅（Festuca rubra var. rubra）对锈病感染（柄锈菌属）的差异表达基因。差异诱导基因被鉴定为拟南芥丝裂原活化蛋白激酶（MAPK）3、大麦杆锈病抗性蛋白Rpg1和菠菜热休克蛋白70（Hsp70）的同源物。通过Northern印迹分析检测了这些基因在锈病感染后的稳态表达水平变化，并通过实时PCR进一步定量。观察到在锈病感染过程中转录本的稳定积累。通过RACE PCR获得了羊茅MPK - 3的全长转录本。其相应的cDNA编码一种预测分子量为42.5 kDa的蛋白质，该蛋白质被映射到同源MAPK的结构模型上以说明相应的MAPK特征基序。本研究首次提供了匍匐紫羊茅中依赖锈病感染的代谢途径的证据。
