Drusenheimer Nadja, Wulf Gerald, Nolte Jessica, Lee Jae Ho, Dev Arvind, Dressel Ralf, Gromoll Jörg, Schmidtke Jörg, Engel Wolfgang, Nayernia Karim
Institute of Human Genetics, University of Göttingen, D-37073 Göttingen.
Soc Reprod Fertil Suppl. 2007;63:69-76.
Germ cells must develop along distinct male or female paths to produce the spermatozoa or oocyte required for sexual reproduction. Male germline stem cells maintain spermatogenesis in the postnatal human testis. Here we show that a small population of bone marrow cells is able to transdifferentiate to male germ cell-like cells. We show expression of early germ cell markers (Oct4, Fragilis, Stella and Vasa) and male germ cell specific markers (Dazl, TSPY, Piwil2 and Stra8) in these cells. Our preliminary findings provide direct evidence that human bone marrow cells can differentiate to putative male germ cells and identify bone marrow as a potential source of male germ cells that could sustain sperm production.
生殖细胞必须沿着不同的雄性或雌性路径发育，以产生有性生殖所需的精子或卵母细胞。雄性生殖系干细胞维持出生后人类睾丸中的精子发生。在此我们表明，一小部分骨髓细胞能够转分化为雄性生殖细胞样细胞。我们在这些细胞中展示了早期生殖细胞标志物（Oct4、Fragilis、Stella和Vasa）以及雄性生殖细胞特异性标志物（Dazl、TSPY、Piwil2和Stra8）的表达。我们的初步发现提供了直接证据，证明人类骨髓细胞可以分化为假定的雄性生殖细胞，并将骨髓确定为可能维持精子产生的雄性生殖细胞的潜在来源。
