采用与钼（VI）进行毛细管内络合的毛细管电泳法同时测定膦酸盐、磷酸盐和二磷酸盐。

Simultaneous determination of phosphonate, phosphate, and diphosphate by capillary electrophoresis using in-capillary complexation with Mo(VI).

作者信息

Himeno Sadayuki, Inazuma Naoko, Kitano Eri

机构信息

Department of Chemistry, Faculty of Science, Kobe University, Nada, Kobe, Japan.

出版信息

J Sep Sci. 2007 May;30(7):1077-81. doi: 10.1002/jssc.200600501.

DOI:10.1002/jssc.200600501

PMID:17566343

Abstract

This study describes the simultaneous determination of phosphonate, phosphate, and diphosphate by CE with direct UV detection, based on in-capillary complexation with Mo(VI). When a mixture of phosphonate, phosphate, and diphosphate was injected into a capillary containing 3.0 mM Mo(VI), 0.05 M malonate buffer (pH 3.0) and 45% v/v CH3CN, three well-defined peaks, due to the migration of the corresponding polyoxomolybdate anions, were separated. The respective calibration graphs were linear in the concentration range of 2 x 10(-6)-2 x 10(-4) M for phosphonate, 1 x 10(-6)-5 x 10(-5) M for phosphate, and 1 x 10(-6)-2 x 10(-4) M for diphosphate; the correlation coefficients were better than 0.9990. The present CE method is successfully applied to the simultaneous determination of phosphonate, phosphate, and diphosphate in tap water.

摘要

本研究描述了基于与钼（VI）的毛细管内络合作用，通过直接紫外检测的毛细管电泳法同时测定膦酸盐、磷酸盐和焦磷酸盐。当将膦酸盐、磷酸盐和焦磷酸盐的混合物注入含有3.0 mM钼（VI）、0.05 M丙二酸盐缓冲液（pH 3.0）和45% v/v乙腈的毛细管中时，由于相应的多氧钼酸盐阴离子的迁移，出现了三个清晰的峰，实现了分离。膦酸盐的校准曲线在2×10⁻⁶ - 2×10⁻⁴ M浓度范围内呈线性，磷酸盐在1×10⁻⁶ - 5×10⁻⁵ M浓度范围内呈线性，焦磷酸盐在1×10⁻⁶ - 2×10⁻⁴ M浓度范围内呈线性；相关系数均优于0.9990。本毛细管电泳方法成功应用于自来水中膦酸盐、磷酸盐和焦磷酸盐的同时测定。