Przybyło Magda, Olzyńska Agnieszka, Han Stanisław, Ozyhar Andrzej, Langner Marek
Wrocław University of Technology, Department of Biomedical Engineering and Measurements, Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland.
Biophys Chem. 2007 Sep;129(2-3):120-5. doi: 10.1016/j.bpc.2007.05.010. Epub 2007 May 24.
There is a constant need for simple, economical and time-efficient methods which allow evaluating a compound's ability to penetrate the biological membrane, one of the key parameters needed to characterize biologically active compounds. In the paper we propose a new method of permeability determination. Instead of detecting the compound's concentration directly, we employ an approach in which the membrane interface is labeled with a fluorescein lipid probe; the probe is sensitive to the presence of charged compounds. The fluorescence intensity changes of the dye permanently attached to both sides of a model lipid bilayer are measured. Specifically, the time course of the fluorescence intensity changes following a rapid induction of a non-equilibrium state of the sample allows the evaluation of the membrane permeability for the compound. The method was validated by the determination of the phenyltin compound's transport through the model phosphatidylcholine unilamellar liposome bilayer.
一直以来都需要简单、经济且省时的方法来评估化合物穿透生物膜的能力，这是表征生物活性化合物所需的关键参数之一。在本文中，我们提出了一种新的渗透率测定方法。我们采用的方法不是直接检测化合物的浓度，而是用荧光素脂质探针标记膜界面；该探针对带电化合物的存在敏感。测量永久附着在模型脂质双分子层两侧的染料的荧光强度变化。具体而言，在快速诱导样品的非平衡状态后，荧光强度变化的时间进程可用于评估化合物的膜渗透率。通过测定苯基锡化合物通过模型磷脂酰胆碱单层脂质体双分子层的转运，验证了该方法。
