Chung Wei-Jen, Shu Hung-Yu, Lu Chi-Yu, Wu Chiu-Yi, Tseng Yi-Hsiung, Tsai Shih-Feng, Lin Chao-Hsiung
Sequencing Core, Genome Research Center, National Yang-Ming University, Taipei, Taiwan.
Proteomics. 2007 Jun;7(12):2047-58. doi: 10.1002/pmic.200600647.
The bacterium Xanthomonas campestris pathovar campestris (XCC) 17 is a local isolate that causes crucifer black rot disease in Taiwan. In this study, its proteome was separated using 2-DE and the well-resolved proteins were excised, trypsin digested, and analyzed by MS. Over 400 protein spots were analyzed and 281 proteins were identified by searching the MS or MS/MS spectra against the proteome database of the closely related XCC ATCC 33913. Functional categorization of the identified proteins matched 141 (50%) proteins to 81 metabolic pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. In addition, we performed a comparative proteome analysis of the pathogenic strain 17 and an avirulent strain 11A to reveal the virulence-related proteins. We detected 22 up-regulated proteins in strain 17 including the degrading enzymes EngXCA, HtrA, and PepA, which had been shown to have a role in pathogenesis in other bacteria, and an anti-host defense protein, Ohr. Thus, further functional studies of these up-regulated proteins with respect to their roles in XCC pathogenicity are suggested.
野油菜黄单胞菌野油菜致病变种（XCC）17是台湾本地分离出的一种可引发十字花科植物黑腐病的细菌。在本研究中，采用双向电泳（2-DE）分离其蛋白质组，对分离良好的蛋白质点进行切取、胰蛋白酶消化，并通过质谱（MS）分析。分析了400多个蛋白质点，通过将质谱或串联质谱（MS/MS）图谱与密切相关的XCC ATCC 33913的蛋白质组数据库进行比对，鉴定出281种蛋白质。对鉴定出的蛋白质进行功能分类，在京都基因与基因组百科全书（KEGG）数据库中，141种（50%）蛋白质与81条代谢途径相匹配。此外，我们对致病菌株17和无毒菌株11A进行了蛋白质组比较分析，以揭示与毒力相关的蛋白质。我们在菌株17中检测到22种上调蛋白，包括降解酶EngXCA、HtrA和PepA，这些酶在其他细菌的致病过程中已被证明发挥作用，还有一种抗宿主防御蛋白Ohr。因此，建议对这些上调蛋白在XCC致病性中的作用进行进一步的功能研究。
