通过同步糖化发酵从苹果渣中生产L-乳酸和低聚化合物：响应面法评估

Production of L-lactic acid and oligomeric compounds from apple pomace by simultaneous saccharification and fermentation: a response surface methodology assessment.

作者信息

Gullón Beatriz, Garrote Gil, Alonso José Luis, Parajó J C

机构信息

Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, 32004 Ourense, Spain.

出版信息

J Agric Food Chem. 2007 Jul 11;55(14):5580-7. doi: 10.1021/jf070442v. Epub 2007 Jun 13.

DOI:10.1021/jf070442v

PMID:17567032

Abstract

The potential of apple pomace for lactic acid production by simultaneous saccharification and fermentation (SSF) was evaluated. The effects of the cellulase to solid ratio (CSR), the liquor to solid ratio (LSR), and the beta-glucosidase to cellulase ratio (BCR) on the kinetics of lactic acid generation were assessed, and a set of mathematical models was developed to reproduce and predict the lactic acid concentration of fermentation broths. Operating at low cellulase and cellobiase charges (1 FPU/g and 0.25 IU/FPU, respectively) and short reaction times (10 h), SSF media containing 27.8 g of lactic acid/L were obtained with a volumetric productivity of 2.78 g/Lh. Material balances showed that the SSF processing of 100 kg of dry apple pomace results in the production of 36.6 kg of lactic acid, 18.3 kg of oligomeric carbohydrates (which can be used as ingredients for functional foods), 8.4 kg of microbial biomass, and 8 kg uronic acids.

摘要

评估了苹果渣通过同步糖化发酵（SSF）生产乳酸的潜力。评估了纤维素酶与固体比例（CSR）、液固比例（LSR）和β-葡萄糖苷酶与纤维素酶比例（BCR）对乳酸生成动力学的影响，并建立了一组数学模型来再现和预测发酵液中的乳酸浓度。在低纤维素酶和纤维二糖酶用量（分别为1 FPU/g和0.25 IU/FPU）以及短反应时间（10小时）下操作，获得了含有27.8 g/L乳酸的SSF培养基，体积产率为2.78 g/L·h。物料平衡表明，对100 kg干苹果渣进行SSF处理可生产36.6 kg乳酸、18.3 kg低聚碳水化合物（可用作功能性食品的成分）、8.4 kg微生物生物量和8 kg糖醛酸。