Manzoor Asma, Qazi Javed Iqbal, Haq Ikram Ul, Mukhtar Hamid, Rasool Akhtar
Institute of Biochemistry and Biotechnology, University of the Punjab, Quaid-i-Azam Campus, Lahore, 54590 Pakistan.
Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan.
J Biol Eng. 2017 May 5;11:17. doi: 10.1186/s13036-017-0059-2. eCollection 2017.
Probiotic bacteria are becoming an important tool for improving human health, controlling diseases and enhancing immune responses. The availability of a cost effective cultivation conditions has profound effect on the efficiency and role of probiotic bacteria. Therefore the current study was conducted with an objective to develop a low cost growth medium for enhancing the biomass production of a bio-therapeutic bacterial strain AS-14. In this work the isolation of AS-14 bacterial strain was carried out from brinjal using cheese whey as a main carbon source. Moreover, the effect of four other nutritional factors besides cheese whey was investigated on the enhanced cell mass production by using response surface methodology (RSM).
The best culture medium contained 60 g/l cheese whey, 15 g/l glucose and 15 g/l corn steep liquor in addition to other minor ingredients and it resulted in maximum dry cell mass (15.41 g/l). The second-order polynomial regression model determined that the maximum cell mass production (16.02 g/l) would be obtained at temperature 40°C and pH 6.2. Comparative studies showed that cultivation using cheese whey and corn steep liquor with other components of the selected medium generated higher biomass with lower cost than that of De Man, Rogosa and Sharpe (MRS) medium under similar cultivation conditions (pH 6.2 and temperature 40°C).
It is evident that the cell biomass of AS-14 was enhanced by low cost cultivation conditions. Moreover, corn steep liquor and ammonium bisulphate were perceived as low-cost nitrogen sources in combination with other components to substitute yeast extract. Of all these factors, cheese whey, corn steep liquor, yeast extract and two operating conditions (temperature and pH) were found to be the most significant parameters. Thus the cost effective medium developed in this research might be used for large-scale commercial application where economics is quite likely important.
益生菌正成为改善人类健康、控制疾病和增强免疫反应的重要工具。具有成本效益的培养条件对益生菌的效率和作用具有深远影响。因此，本研究旨在开发一种低成本的生长培养基，以提高生物治疗菌株AS-14的生物量产量。在这项工作中，以奶酪乳清作为主要碳源，从茄子中分离出AS-14菌株。此外，还使用响应面法（RSM）研究了除奶酪乳清外的其他四种营养因素对提高细胞质量产量的影响。
最佳培养基除其他微量成分外，还含有60 g/l奶酪乳清、15 g/l葡萄糖和15 g/l玉米浆，其干细胞质量最高（15.41 g/l）。二阶多项式回归模型确定，在温度40°C和pH 6.2时可获得最大细胞质量产量（16.02 g/l）。比较研究表明，在相似培养条件（pH 6.2和温度40°C）下，使用奶酪乳清和玉米浆与所选培养基的其他成分进行培养，比德氏、罗氏和夏普（MRS）培养基能以更低成本产生更高的生物量。
显然，低成本培养条件提高了AS-14的细胞生物量。此外，玉米浆和硫酸氢铵被视为低成本氮源，可与其他成分结合替代酵母提取物。在所有这些因素中，奶酪乳清、玉米浆、酵母提取物和两个操作条件（温度和pH）被发现是最显著的参数。因此，本研究开发的具有成本效益的培养基可用于经济因素很可能很重要的大规模商业应用。
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