Patel Shyam, Kurpinski Kyle, Quigley Ryan, Gao Hongfeng, Hsiao Benjamin S, Poo Mu-Ming, Li Song
Joint Graduate Program in Bioengineering, University of California San Francisco, University of California Berkeley, California 94720-1762, USA.
Nano Lett. 2007 Jul;7(7):2122-8. doi: 10.1021/nl071182z. Epub 2007 Jun 14.
Biodegradable nanofibers have tremendous potential for tissue repair. However, the combined effects of nanofiber organization and immobilized bioactive factors on cell guidance are not well understood. In this study, we developed aligned and bioactive nanofibrous scaffolds by immobilizing extracellular matrix protein and growth factor onto nanofibers, which simulated the physical and biochemical properties of native matrix fibrils. The aligned nanofibers significantly induced neurite outgrowth and enhanced skin cell migration during wound healing compared to randomly oriented nanofibers. Furthermore, the immobilized biochemical factors (as efficient as soluble factors) synergized with aligned nanofibers to promote highly efficient neurite outgrowth but had less effect on skin cell migration. This study shed light on the relative importance of nanotopography and chemical signaling in the guidance of different cell behavior.
可生物降解的纳米纤维在组织修复方面具有巨大潜力。然而，纳米纤维的排列方式和固定化生物活性因子对细胞引导的综合作用尚未得到充分理解。在本研究中，我们通过将细胞外基质蛋白和生长因子固定在纳米纤维上，开发出了排列有序且具有生物活性的纳米纤维支架，该支架模拟了天然基质原纤维的物理和生化特性。与随机取向的纳米纤维相比，排列有序的纳米纤维在伤口愈合过程中显著诱导了神经突生长并增强了皮肤细胞迁移。此外，固定化的生化因子（与可溶性因子效果相当）与排列有序的纳米纤维协同作用，促进了高效的神经突生长，但对皮肤细胞迁移的影响较小。这项研究揭示了纳米拓扑结构和化学信号在引导不同细胞行为方面的相对重要性。
