Chiou Wen-Fei, Don Ming-Jaw
National Research Institute of Chinese Medicine, Taipei, Taiwan, ROC.
Life Sci. 2007 Jun 20;81(2):109-14. doi: 10.1016/j.lfs.2007.04.028. Epub 2007 May 6.
We evaluated the anti-inflammatory effects of cryptotanshinone and tanshinone IIA, two major tanshinones isolated from Salvia miltiorrhiza, on chemoattractant-induced cell migration in RAW264.7 macrophages. Results showed that cryptotanshinone inhibited cell migration toward complement 5a (C5a) and macrophage inflammatory protein-1alpha (MIP-1alpha) in a concentration-dependent manner. In contrast, tanshinone IIA displayed less or even no effect on cell migration evoked by these chemoattractants. Both C5a- and MIP-1alpha-induced migration were clearly inhibited by cytochalasin B (an inhibitor of actin polymerization), but not by colchicine (an inhibitor of microtubule polymerization). Fluorescence staining demonstrated that cryptotanshinone as well as cytochalasin B, effectively reversed cell polarization and filopodia extension induced by both chemoattractants. Furthermore, C5a-evoked increase in F-actin fluorescence intensity was significantly suppressed by cryptotanshinone. Based on these observations, we suggest that cryptotanshinone exerts anti-migrating activity possibly by impeding F-actin polymerization and filopodia formation.
我们评估了隐丹参酮和丹参酮IIA这两种从丹参中分离出的主要丹参酮对RAW264.7巨噬细胞中趋化因子诱导的细胞迁移的抗炎作用。结果表明，隐丹参酮以浓度依赖的方式抑制细胞向补体5a（C5a）和巨噬细胞炎性蛋白-1α（MIP-1α）的迁移。相比之下，丹参酮IIA对这些趋化因子引起的细胞迁移作用较小甚至没有作用。细胞松弛素B（一种肌动蛋白聚合抑制剂）可明显抑制C5a和MIP-1α诱导的迁移，但秋水仙碱（一种微管聚合抑制剂）则无此作用。荧光染色表明，隐丹参酮以及细胞松弛素B可有效逆转两种趋化因子诱导的细胞极化和丝状伪足延伸。此外，隐丹参酮可显著抑制C5a引起的F-肌动蛋白荧光强度增加。基于这些观察结果，我们认为隐丹参酮可能通过阻碍F-肌动蛋白聚合和丝状伪足形成发挥抗迁移活性。
