Isobe Tomohiko, Ramu Karri, Kajiwara Natsuko, Takahashi Shin, Lam Paul K S, Jefferson Thomas A, Zhou Kaiya, Tanabe Shinsuke
Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577, Japan.
Mar Pollut Bull. 2007 Aug;54(8):1139-45. doi: 10.1016/j.marpolbul.2007.04.017. Epub 2007 Jun 12.
A diastereoisomer (alpha, beta, and gamma) specific analytical method for measuring hexabromocyclododecanes (HBCDs) was developed using liquid chromatography-tandem mass spectrometry (LC-MS-MS). The method developed was applied to analyze blubber of small cetaceans to divulge the distribution and temporal variation of HBCDs in the Asian marine environment. HBCDs were detected in all the blubber samples of finless porpoises (Neophocaena phocaenoides) and Indo-Pacific humpback dolphins (Sousa chinensis) collected from the South China Sea during 1990-2001. Concentrations of HBCDs were higher in humpback dolphins (31-380 ng/g lipid) than in finless porpoises (4.7-55 ng/g lipid), which can be attributed to habitat differences. Average concentrations of alpha-HBCD in finless porpoises increased from 9.5 ng/g lipid in 1990 to 35 ng/g lipid in 2000/01. alpha-HBCD was predominant among the three isomers with some exceptions in finless porpoises collected in 1990. This is the first report on HBCDs contamination in marine mammals from Southeast Asia.
采用液相色谱 - 串联质谱法（LC-MS-MS）开发了一种用于测定六溴环十二烷（HBCD）非对映异构体（α、β和γ）的特定分析方法。所开发的方法应用于分析小型鲸类动物的鲸脂，以揭示亚洲海洋环境中HBCD的分布和时间变化。在1990 - 2001年期间从中国南海采集的所有江豚（Neophocaena phocaenoides）和印太驼海豚（Sousa chinensis）的鲸脂样本中均检测到了HBCD。印太驼海豚体内HBCD的浓度（31 - 380 ng/g脂质）高于江豚（4.7 - 55 ng/g脂质），这可能归因于栖息地差异。江豚体内α-HBCD的平均浓度从1990年的9.5 ng/g脂质增加到2000/01年的35 ng/g脂质。在这三种异构体中，α-HBCD占主导地位，但1990年采集的江豚样本中有一些例外情况。这是关于东南亚海洋哺乳动物中HBCD污染的首次报告。
