Sjöholm A
Department of Medical Cell Biology, Uppsala University, Sweden.
FEBS Lett. 1991 Dec 9;294(3):257-60. doi: 10.1016/0014-5793(91)81442-b.
Long-term effects of the protein kinase C activating phorbol ester, TPA, on pancreatic beta-cell proliferation and insulin production were investigated. It was found that beta-cell replication and long-term insulin secretion were enhanced in TPA-treated islets. This was not accompanied by a corresponding increase in (pro)insulin biosynthesis, presumably contributing to the lowered islet insulin content. TPA also increased islet polyamine content but when this increase was prevented by blocking polyamine synthesis, DNA replication and insulin secretion remained elevated. These findings indicate that TPA stimulates beta-cell replication and insulin secretion and suggest a stimulatory role for protein kinase C, but not for polyamines, in these processes.
研究了蛋白激酶C激活剂佛波酯（TPA）对胰腺β细胞增殖和胰岛素产生的长期影响。结果发现，经TPA处理的胰岛中β细胞复制和长期胰岛素分泌增强。这并未伴随着（前）胰岛素生物合成相应增加，可能导致胰岛胰岛素含量降低。TPA还增加了胰岛多胺含量，但当通过阻断多胺合成来阻止这种增加时，DNA复制和胰岛素分泌仍保持升高。这些发现表明，TPA刺激β细胞复制和胰岛素分泌，并提示蛋白激酶C而非多胺在这些过程中起刺激作用。
