[两种饮食对接受脾脏手术大鼠血脂的影响]

[Effects of two types of diet on plasma lipids in rats submitted to splenic surgery].

作者信息

Paulo Isabel Cristina Andreatta Lemos, Paulo Danilo Nagib Salomão, Kalil Mitre, Guerra Alvino Jorge, Guerzet Eduardo Almeida, Silva Alcino Lázaro da

机构信息

Laboratório de Fundamentos da Cirurgia, Escola Superior de Ciências, Santa Casa de Misericórdia de Vitória, ES.

出版信息

Rev Assoc Med Bras (1992). 2007 Mar-Apr;53(2):171-7. doi: 10.1590/s0104-42302007000200024.

DOI:10.1590/s0104-42302007000200024

PMID:17568924

Abstract

OBJECTIVE

The objective was to verify the effect of total splenectomy and ligature of the splenic vessels on the plasma lipid levels in rats fed a control diet or a diet supplemented with 2.5% cholesterol.

METHODS

Ninety-nine male Wistar rats weighting between 273 and 427 g (arithmetic mean: 349.46 +/-35.54) were randomly divided into four groups: group 1- control (N=19), nonoperated; group 2 - sham (N=19), laparotomy with spleen manipulation; group 3 -TS (N=31), total splenectomy; group 4 (N=30) simultaneous ligature of the splenic artery and vein. Blood was collected from all animals for determination of plasma lipids at the beginning of the experiment. Rats were than divided into two subgroups receiving either control diet (subgroup A) or diet supplemented with 2.5% cholesterol (subgroup B). Another blood sample was collected 90 days later for a new determination.

RESULTS

No significant difference in plasma lipids between the four groups was observed at the beginning (p>0.05). In subgroups 1A, 2A and 4A, there was no significant difference in the values of HDL, VLDL and triglycerides, except LDL that dropped significantly in subgroup 1A and 2A (p<0.05) and not significantly in subgroup 4A. In subgroup 1B, 2B and 4B there was a significant increase in late postoperative plasma total cholesterol, LDL, VLDL (p<0.05) and a not significant increase of HDL. In total splenectomy, at late postoperative there was a significant increase of cholesterol, LDL, VLDL and triglycerides, especially in subgroup B. In group 3 the cholesterol, LDL and triglycerides were more significant than in groups 1, 2 and 4.

CONCLUSION

Total splenectomy caused a more significant increase of cholesterol, LDL, VLDL and triglycerides in rats fed a cholesterol diet. The simultaneous ligature of vein and artery prevented alterations in plasma lipids observed in rats submitted to total splenectomy and maintained lipid levels similar to those of the control and sham groups.

摘要

目的

验证全脾切除术及脾血管结扎对喂食对照饮食或添加2.5%胆固醇饮食的大鼠血浆脂质水平的影响。

方法

99只体重在273至427克之间（算术平均值：349.46±35.54）的雄性Wistar大鼠被随机分为四组：第1组 - 对照组（N = 19），未手术；第2组 - 假手术组（N = 19），行剖腹术并对脾脏进行操作；第3组 - 全脾切除术组（N = 31）；第4组（N = 30），同时结扎脾动脉和脾静脉。在实验开始时从所有动物采集血液以测定血浆脂质。然后将大鼠分为接受对照饮食（A亚组）或添加2.5%胆固醇饮食（B亚组）的两个亚组。90天后采集另一份血样进行新的测定。

结果

实验开始时，四组之间血浆脂质无显著差异（p>0.05）。在1A、2A和4A亚组中，高密度脂蛋白（HDL）、极低密度脂蛋白（VLDL）和甘油三酯的值无显著差异，除了低密度脂蛋白（LDL）在1A和2A亚组中显著下降（p<0.05），在4A亚组中无显著下降。在1B、2B和4B亚组中，术后晚期血浆总胆固醇、LDL、VLDL显著升高（p<0.05），HDL无显著升高。在全脾切除术中，术后晚期胆固醇、LDL、VLDL和甘油三酯显著升高，尤其是在B亚组中。第3组中的胆固醇、LDL和甘油三酯比第1、2和4组更显著。