González Ranulfo, Wilkerson Richard, Suárez Marco Fidel, García Felipe, Gallego Gerardo, Cárdenas Heiber, Posso Carmen Elisa, Duque Myriam Cristina
Facultad de Ciencias y Facultad de Salud, Universidad del Valle, Cali, Colombia.
Mem Inst Oswaldo Cruz. 2007 Jun;102(3):255-62. doi: 10.1590/s0074-02762007005000037.
The genetic variation and population structure of three populations of Anopheles darlingi from Colombia were studied using random amplified polymorphic markers (RAPDs) and amplified fragment length polymorphism markers (AFLPs). Six RAPD primers produced 46 polymorphic fragments, while two AFLP primer combinations produced 197 polymorphic fragments from 71 DNA samples. Both of the evaluated genetic markers showed the presence of gene flow, suggesting that Colombian An. darlingi populations are in panmixia. Average genetic diversity, estimated from observed heterozygosity, was 0.374 (RAPD) and 0.309 (AFLP). RAPD and AFLP markers showed little evidence of geographic separation between eastern and western populations; however, the F ST values showed high gene flow between the two western populations (RAPD: F ST = 0.029; Nm: 8.5; AFLP: F ST = 0.051; Nm: 4.7). According to molecular variance analysis (AMOVA), the genetic distance between populations was significant (RAPD:phiST = 0.084; AFLP:phiST = 0.229, P < 0.001). The F ST distances and AMOVAs using AFLP loci support the differentiation of the Guyana biogeographic province population from those of the Chocó-Magdalena. In this last region, Chocó and Córdoba populations showed the highest genetic flow.
利用随机扩增多态性标记（RAPD）和扩增片段长度多态性标记（AFLP）对来自哥伦比亚的三个达林按蚊种群的遗传变异和种群结构进行了研究。6条RAPD引物产生了46个多态性片段，而2对AFLP引物组合从71个DNA样本中产生了197个多态性片段。两种评估的遗传标记均显示存在基因流，这表明哥伦比亚的达林按蚊种群处于随机交配状态。根据观察到的杂合度估计的平均遗传多样性，RAPD为0.374，AFLP为0.309。RAPD和AFLP标记几乎没有显示出东部和西部种群之间存在地理隔离的证据；然而，FST值显示两个西部种群之间存在高基因流（RAPD：FST = 0.029；Nm：8.5；AFLP：FST = 0.051；Nm：4.7）。根据分子方差分析（AMOVA），种群之间的遗传距离显著（RAPD：phiST = 0.084；AFLP：phiST = 0.229，P < 0.001）。使用AFLP位点的FST距离和AMOVA支持圭亚那生物地理省种群与乔科 - 马格达莱纳种群的分化。在最后这个区域，乔科和科尔多瓦种群显示出最高的基因流。
