[冻融损伤山羊精子的质膜完整性]
[Freezing-thawing damages plasma membrane integrity of goat spermatozoa].
作者信息
Zhang Xu-gang, Zhao Yong-ju, Li Zhou-quan
机构信息
College of Animal, Veterinary and Aquacultural Sciences, Chongqing Key Laboratory of Forage & Herbivore, Southwest University, Chongqing 400716, China.
出版信息
Zhonghua Nan Ke Xue. 2007 May;13(5):403-6.
OBJECTIVE
To evaluate the plasma membrane integrity and morphology of fresh and frozen goat spermatozoa.
METHODS
The ejaculates of three male goats were obtained by the artificial vagina method of collection and the rates of sperm abnormality and acrosome integrity were detected after freezing-thawing processing. The plasma membrane integrity of the fresh and frozen-thawed goat spermatozoa was evaluated with a combination of fluorescent probes, carboxyfluorescein diacetate and propidium iodide.
RESULTS
The freezing-thawing process significantly influenced the viability and integrity of the spermatozoa ([74.43 +/- 13.78]% vs. [46.25 +/- 2.69]%; [64.26 +/- 7.03]% vs. [6.27 +/- 2.90]%, P < 0.01). The results showed differences in acrosome integrity rate between the fresh and frozen samples ([80.77 +/- 10.70]% vs. [58.42 +/- 18.05]% , P < 0.05).
CONCLUSION
The freezing-thawing process significantly reduces sperm viability and acrosome integrity and seriously damages the plasma membrane integrity.
目的
评估新鲜及冷冻山羊精子的质膜完整性和形态。
方法
通过人工阴道采精法获取三只雄性山羊的精液，经冻融处理后检测精子畸形率和顶体完整性。采用荧光探针羧基荧光素二乙酸酯和碘化丙啶组合评估新鲜及冻融后山羊精子的质膜完整性。
结果
冻融过程显著影响精子的活力和完整性（[74.43±13.78]%对[46.25±2.69]%；[64.26±7.03]%对[6.27±2.90]%，P<0.01）。结果显示新鲜样本与冷冻样本之间顶体完整性率存在差异（[80.77±10.70]%对[58.42±18.05]%，P<0.05）。
结论
冻融过程显著降低精子活力和顶体完整性，并严重损害质膜完整性。
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