谷胱甘肽S-转移酶T1基因多态性与特发性无精子症和少精子症的相关性
[Genetic polymorphism of glutathione S-transferase T1 associated with idiopathic azoospermia and oligospermia].
作者信息
Wu Qi-fei, Xing Jun-ping, Sun Jian-hua, Xue Wei, Wang Xin-yang, Jin Xiao-juan
机构信息
Department of Urology, the First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
出版信息
Zhonghua Nan Ke Xue. 2007 May;13(5):407-10.
OBJECTIVE
To investigate the association of glutathioneS-transferase T (GSTT1) gene polymorphism with azoospermia and oligospermia.
METHODS
Semen samples from 34 patients with idiopathic azoospermia, 40 patients with idiopathic oligospermia and 53 healthy controls with normal sperm concentration and motility were assessed according to the standards of WHO. The GSTT1 genotypes were identified by multiplex polymerase chain reaction (PCR) with peripheral blood DNA samples.
RESULTS
The frequencies of null GSTTI genotypes in the patients with idiopathic azoospermia, idiopathic oligospermia and the healthy controls were 76.5%, 72.5% and 49.1%, respectively. There was a significant association between the null alleles of GSTT1 and idiopathic azoospermia (odds ratio 3.13, 95% CI 1.20-8.16, P = 0.020) and idiopathic oligospermia (odds radio 2.53, 95% CI 1.06-6.11, P = 0.038).
CONCLUSION
The null alleles of GSTTI are a strong predisposing risk factor for idiopathic azoospermia and oligospermia.
目的
探讨谷胱甘肽S-转移酶T（GSTT1）基因多态性与无精子症和少精子症的关联。
方法
根据世界卫生组织标准，对34例特发性无精子症患者、40例特发性少精子症患者以及53例精子浓度和活力正常的健康对照者的精液样本进行评估。采用多重聚合酶链反应（PCR）对外周血DNA样本进行GSTT1基因型鉴定。
结果
特发性无精子症患者、特发性少精子症患者和健康对照者中GSTT1无效基因型的频率分别为76.5%、72.5%和49.1%。GSTT1的无效等位基因与特发性无精子症（优势比3.13，95%可信区间1.20 - 8.16，P = 0.020）和特发性少精子症（优势比2.53，95%可信区间1.06 - 6.11，P = 0.038）之间存在显著关联。
结论
GSTT1的无效等位基因是特发性无精子症和少精子症的一个强烈的易感危险因素。
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