Patiño-García Brenda, Arroyo Carlos, Rangel-Villalobos Hector, Soto-Vega Elena, Velarde-Félix Jose Salvador, Gabilondo Fernando, Sandoval-Ramirez Lucila, Figuera Luis Eduardo
División de Genética, Centro de Investigación Biomédica de Occidente-Instituto Mexicano del Seguro Social, Guadalajara.
Rev Invest Clin. 2007 Jan-Feb;59(1):25-31.
Prostate cancer (PCa) is a worldwide health issue, because of its high incidence and mortality. Its etiology is complex and includes certain risk factors such as age, hormonal status, ethnic origin and family history of PCa. Genetic predisposition is proposed as a major risk factor and there are several controversial reports on the association of PCa and gene polymorphism such as the receptors of the androgen receptor (AR) and the vitamin D (VDR). Objective. To evaluate the CAG triplet repeats in the first exon of the AR and polymorphisms in the restriction site Taql in the VDR in Mexicans with PCa.
A total of 68 Mexicans with histopathological diagnosis of PCa and 48 healthy Mexican with normal prostate-specific antigen and rectal exam where included. 10ml of peripheral blood were extracted to isolate DNA and the polymorphisms were evaluated with specific primers for the AR and VDR.
The allelic and genetic distributions of the AR and VDR polymorphisms were consistent with the Hardy-Weinberg equilibrium, and there were no statistical differences between the PCa patients and controls (p > 0.05). However, there was a statistical difference between the number of CAG repeats in younger patients with PCa compared to controls (p = 0.045) but when the young patient group was compared versus the elder group there was not stadistically difference (p = 0.085), but the results showed a tendency towards less repetitions of CAG in elder patients. Concerning the VDR, when we analyzed the patients with PCa and a bad pathological prognosis they had a less frequent genotype of TT (p = 0.03).
Our results suggest an association between the VDR and AR gene polymorphisms, and the histopathological score and age at diagnosis in Mexican patients with PCa, respectively. However, it is important to confirm these results in a larger scale study.
前列腺癌（PCa）是一个全球性的健康问题，因其高发病率和死亡率。其病因复杂，包括某些风险因素，如年龄、激素状态、种族起源和前列腺癌家族史。遗传易感性被认为是一个主要风险因素，并且有一些关于前列腺癌与基因多态性（如雄激素受体（AR）和维生素D受体（VDR））关联的有争议的报告。目的：评估患有前列腺癌的墨西哥人中AR第一外显子的CAG三联体重复以及VDR中限制性位点TaqI的多态性。
总共纳入了68名经组织病理学诊断为前列腺癌的墨西哥人和48名前列腺特异性抗原和直肠检查正常的健康墨西哥人。抽取10毫升外周血以分离DNA，并使用针对AR和VDR的特异性引物评估多态性。
AR和VDR多态性的等位基因和基因分布符合哈迪-温伯格平衡，前列腺癌患者与对照组之间无统计学差异（p>0.05）。然而，与对照组相比，年轻前列腺癌患者的CAG重复次数存在统计学差异（p = 0.045），但当年轻患者组与老年组进行比较时，无统计学差异（p = 0.085），但结果显示老年患者的CAG重复次数有减少的趋势。关于VDR，当我们分析前列腺癌且病理预后不良的患者时，他们的TT基因型频率较低（p = 0.03）。
我们的结果分别提示VDR和AR基因多态性与墨西哥前列腺癌患者的组织病理学评分和诊断年龄之间存在关联。然而，在更大规模的研究中证实这些结果很重要。
