Moreau John W, Weber Peter K, Martin Michael C, Gilbert Benjamin, Hutcheon Ian D, Banfield Jillian F
Department of Earth and Planetary Science, University of California Berkeley, Berkeley, CA 94720, USA.
Science. 2007 Jun 15;316(5831):1600-3. doi: 10.1126/science.1141064.
High-spatial-resolution secondary ion microprobe spectrometry, synchrotron radiation-based Fourier-transform infrared spectroscopy, and polyacrylamide gel analysis demonstrated the intimate association of proteins with spheroidal aggregates of biogenic zinc sulfide nanocrystals, an example of extracellular biomineralization. Experiments involving synthetic zinc sulfide nanoparticles and representative amino acids indicated a driving role for cysteine in rapid nanoparticle aggregation. These findings suggest that microbially derived extracellular proteins can limit the dispersal of nanoparticulate metal-bearing phases, such as the mineral products of bioremediation, that may otherwise be transported away from their source by subsurface fluid flow.
高空间分辨率二次离子微探针光谱法、基于同步辐射的傅里叶变换红外光谱法以及聚丙烯酰胺凝胶分析表明，蛋白质与生物源硫化锌纳米晶体的球状聚集体紧密相关，这是细胞外生物矿化的一个例子。涉及合成硫化锌纳米颗粒和代表性氨基酸的实验表明，半胱氨酸在纳米颗粒快速聚集过程中起驱动作用。这些发现表明，微生物衍生的细胞外蛋白质可以限制含纳米颗粒的金属相的扩散，比如生物修复的矿产物，否则这些物质可能会通过地下流体流动从其源头被带走。
