Hoskins Roger A, Carlson Joseph W, Kennedy Cameron, Acevedo David, Evans-Holm Martha, Frise Erwin, Wan Kenneth H, Park Soo, Mendez-Lago Maria, Rossi Fabrizio, Villasante Alfredo, Dimitri Patrizio, Karpen Gary H, Celniker Susan E
Department of Genome and Computational Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Science. 2007 Jun 15;316(5831):1625-8. doi: 10.1126/science.1139816.
Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the generation of 15 Mb of finished or improved heterochromatic sequence with the use of available clone resources and assembly methods. We also constructed a bacterial artificial chromosome-based physical map that spans 13 Mb of the pericentromeric heterochromatin and a cytogenetic map that positions 11 Mb in specific chromosomal locations. We have approached a complete assembly and mapping of the nonsatellite component of Drosophila heterochromatin. The strategy we describe is also applicable to generating substantially more information about heterochromatin in other species, including humans.
由于异染色质中存在重复DNA，大多数后生动物和植物的基因组序列并不完整。黑腹果蝇的异染色质区域包含2000万个碱基（Mb）的序列，适合进行图谱绘制、序列组装和完善。我们描述了利用现有的克隆资源和组装方法生成15 Mb已完成或改进的异染色质序列。我们还构建了一个基于细菌人工染色体的物理图谱，该图谱跨越13 Mb的着丝粒周围异染色质，以及一个细胞遗传图谱，将11 Mb定位在特定的染色体位置。我们已接近完成果蝇异染色质非卫星成分的组装和图谱绘制。我们所描述的策略也适用于生成关于其他物种（包括人类）异染色质的更多信息。
