Knorr M, Steuhl K P, Wunderlich K, Raphael B, Thiel H J
Universitäts-Augenklinik, Tübingen, Bundesrepublik Deutschland.
Fortschr Ophthalmol. 1991;88(5):434-7.
Platelet-derived growth factor (PDGF) is one of numerous growth factors regulating development of the ocular lens and its component parts. Although little is known about its site of action on the cell and the mechanisms of this confirmed mitogenic action, a close connection has been assumed between the influence of PDGF on both the lens epithelium and intracellular calcium homeostasis. The present study therefore examined the influence of PDGF on the concentration of intracellular free calcium [( Ca2+]i) in primary cultivated bovine epithelial cells. A dose-dependent [Ca2+]i increase of up to 300% followed stimulation with PDGF (5-50 ng/ml) in a medium containing Ca2+. [Ca2+]i also rose significantly in Ca(2+)-free medium, indicating mobilization from intracellular storage.
血小板衍生生长因子（PDGF）是众多调节晶状体及其组成部分发育的生长因子之一。尽管对于其在细胞上的作用位点以及这种已确认的促有丝分裂作用的机制了解甚少，但人们认为PDGF对晶状体上皮细胞和细胞内钙稳态的影响之间存在密切联系。因此，本研究检测了PDGF对原代培养的牛上皮细胞内游离钙浓度[Ca2+]i的影响。在含有钙离子的培养基中，用PDGF（5 - 50 ng/ml）刺激后，[Ca2+]i呈剂量依赖性增加，最高可达300%。在无钙培养基中，[Ca2+]i也显著升高，表明钙从细胞内储存库中释放出来。
