家牛体内的内源性类JSRV前病毒：序列与转录本分析

Endogenous JSRV-like proviruses in domestic cattle: analysis of sequences and transcripts.

作者信息

Morozov V A, Morozov A V, Lagaye S

机构信息

Robert Koch-Institut, Retrovirology, 13353 Berlin, Germany.

出版信息

Virology. 2007 Oct 10;367(1):59-70. doi: 10.1016/j.virol.2007.05.018. Epub 2007 Jun 13.

DOI:10.1016/j.virol.2007.05.018

PMID:17570457

Abstract

Jaagsiekte retrovirus is an exogenous (exJSRV) beta-retrovirus with a simple genome. It causes lower airway epithelial cell tumors in small ruminants. Endogenous (enJSRV) counterparts of exJSRV are present in different copy numbers in numerous Bovidae family members. This work has focused on enJSRV in Simmental (Germany) and Limousine (France) beef breeds of domestic cattle and domestic goat. Of the enJSRV sequences in cattle, the orf-x sequences were about 99% identical, the LTR sequences were about 97% identical and the env sequences were nearly 95% identical to the corresponding endogenous sequences in sheep. A significant polymorphism of the proviral sequences between the cattle breeds was noted. Clonal analyses of the amplicons suggest two enJSRV proviruses in cattle genome. The endogenous sequences revealed in goat were closer to enzootic nasal tumor virus (ENTV) from goat rather than to enJSRV from sheep. The expression of enJSRV in cattle was partial (env only) and detected exclusively in bone marrow.

摘要

绵羊肺腺瘤逆转录病毒是一种具有简单基因组的外源性（exJSRV）β逆转录病毒。它会导致小型反刍动物的下呼吸道上皮细胞瘤。exJSRV的内源性（enJSRV）对应物在众多牛科动物家族成员中以不同的拷贝数存在。这项工作聚焦于德国西门塔尔牛和法国利木赞牛以及家养山羊中的enJSRV。在牛的enJSRV序列中，orf-x序列与绵羊中相应的内源性序列约99%相同，LTR序列约97%相同，env序列近95%相同。注意到牛品种之间前病毒序列存在显著多态性。扩增子的克隆分析表明牛基因组中有两种enJSRV前病毒。在山羊中发现的内源性序列与山羊的地方流行性鼻肿瘤病毒（ENTV）更接近，而不是与绵羊的enJSRV接近。enJSRV在牛中的表达是部分性的（仅env），且仅在骨髓中检测到。