Scillitani Giovanni, Zizza Sara, Liquori Giuseppa Esterina, Ferri Domenico
Dipartimento di Zoologia, Laboratorio di Istologia e Anatomia comparata, Università degli Studi di Bari, via Orabona 4/a, I-70125 Bari, Italy.
Acta Histochem. 2007;109(5):347-57. doi: 10.1016/j.acthis.2007.02.010. Epub 2007 Jun 13.
Mucins in the gastrointestinal tract of Rhinolophus ferrumequinum were investigated by histochemistry and lectin histochemistry to evaluate morphofunctional variations of different regions and their possible physiological and evolutionary implications. Histochemical methods included periodic acid-Schiff (PAS), Alcian blue (AB) at pH 2.5 and 1.0 and high-iron-diamine AB pH 2.5. Binding of lectins Con A, DBA, WGA, LTA, LFA, PNA and SBA; LFA, PNA and SBA with prior sialidase treatment; and paradoxical Con A were evaluated. The oesophagus lacked glands. The stomach was divided into a short cardias, a wide fundus and a brief pylorus. The surface muciparous cells secreted sulpho- and sialomucins with N-acetylgalactosamine (GalNAc) residues, N-acetyllactosamine and (beta1,4 N-acetylglucosamine)(n) chains. Towards the pylorus, N-acetylgalactosamine residues disappeared and acidity decreased. Cardiac glands, neck cells in the fundic glands, pyloric and duodenal Brunner's glands all shared neutral, stable class-III mucins, mainly with N-acetylgalactosamine sequences. The intestine was divided into a duodenum, a jejuno-ileum and a short rectum. The goblet cells produced sulpho- and sialomucins with sialylated N-acetylgalactosamine sequences, (beta1,4 N-acetylglucosamine)(n) and N-acetyllactosamine, whose sialylation increased towards the rectum. The main features of the mucins are probably associated with the requirements of fast absorption and food passage and in protection against mechanical and pathogenic injuries.
通过组织化学和凝集素组织化学方法对马铁菊头蝠胃肠道中的黏蛋白进行了研究，以评估不同区域的形态功能变化及其可能的生理和进化意义。组织化学方法包括过碘酸希夫反应（PAS）、pH 2.5和1.0的阿尔辛蓝（AB）以及高铁二胺AB pH 2.5。评估了凝集素刀豆球蛋白A（Con A）、麦胚凝集素（DBA）、小麦胚凝集素（WGA）、脂多糖（LTA）、荆豆凝集素（LFA）、花生凝集素（PNA）和大豆凝集素（SBA）的结合情况；LFA、PNA和SBA经唾液酸酶预处理后的结合情况；以及反常Con A的结合情况。食管无腺体。胃分为短贲门部、宽底部和短幽门部。表面黏液分泌细胞分泌含N-乙酰半乳糖胺（GalNAc）残基、N-乙酰乳糖胺和（β1,4 N-乙酰葡糖胺）（n）链的硫酸化和唾液酸化黏蛋白。靠近幽门处，N-乙酰半乳糖胺残基消失，酸度降低。贲门腺、胃底腺颈部细胞、幽门腺和十二指肠布伦纳腺均含有中性、稳定的Ⅲ类黏蛋白，主要含有N-乙酰半乳糖胺序列。肠道分为十二指肠、空回肠和短直肠。杯状细胞产生含唾液酸化N-乙酰半乳糖胺序列、（β1,4 N-乙酰葡糖胺）（n）和N-乙酰乳糖胺的硫酸化和唾液酸化黏蛋白，其唾液酸化程度向直肠方向增加。黏蛋白的主要特征可能与快速吸收和食物通过的需求以及抵御机械和病原体损伤有关。
