Akashi Tetsuyuki, Yamori Takao
Division of Molecular Pharmacology, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo, Japan.
Proteomics. 2007 Jul;7(14):2350-4. doi: 10.1002/pmic.200700157.
A novel SELDI-TOF MS-based method for analyzing phosphoproteins was developed using a series of recombinant wild-type and mutant ribosomal P2 proteins. We demonstrated that the phosphorylation status of the overexpressed proteins in cells was easily and rapidly confirmed using this method. The ribosomal P2 protein contained two phosphorylation sites, which were sequentially phosphorylated in vivo. We also quantitatively detected the phosphoprotein by using SELDI-TOF MS.
利用一系列重组野生型和突变型核糖体P2蛋白，开发了一种基于表面增强激光解吸电离飞行时间质谱（SELDI-TOF MS）的新型磷蛋白分析方法。我们证明，使用该方法可以轻松快速地确认细胞中过表达蛋白的磷酸化状态。核糖体P2蛋白含有两个磷酸化位点，它们在体内依次被磷酸化。我们还使用SELDI-TOF MS对磷蛋白进行了定量检测。
