Zhang Xumin, Shi Liang, Shu Shaokung, Wang Yuan, Zhao Kang, Xu Ningzhi, Liu Siqi, Roepstorff Peter
Beijing Genomics institute, Chinese Academy of Science, Beijing, China.
Proteomics. 2007 Jul;7(14):2340-9. doi: 10.1002/pmic.200600184.
An improved method for sample preparation for MALDI-MS and MS/MS using AnchorChip targets is presented. The method, termed the SMW method (sample, matrix wash), results in better sensitivity for peptide mass fingerprinting as well as for sequencing by MS/MS than previously published methods. The method allows up-concentration and desalting directly on the mass spectrometric target and should be amenable for automation. A draw back caused by extensive oxidation of methionine and tryptophan in the SMW method can be alleviated by the addition of n-octyl glucopyranoside and DTT to the sample solution. The method was validated for protein identification from a 2-DE based liver proteome study. The SMW method resulted in identification of many more proteins and in most cases with a better score than the previously published methods.
本文介绍了一种使用AnchorChip靶板进行MALDI-MS和MS/MS样品制备的改进方法。该方法称为SMW方法（样品、基质洗涤），与以前发表的方法相比，在肽质量指纹分析以及MS/MS测序方面具有更高的灵敏度。该方法允许在质谱靶板上直接进行浓缩和脱盐，并且应该适合自动化操作。通过向样品溶液中添加正辛基吡喃葡萄糖苷和二硫苏糖醇（DTT），可以缓解SMW方法中蛋氨酸和色氨酸广泛氧化所导致的一个缺点。该方法已在基于二维电泳的肝脏蛋白质组研究中用于蛋白质鉴定的验证。SMW方法鉴定出了更多的蛋白质，并且在大多数情况下，得分比以前发表的方法更高。
