Nash J R, McLaughlin P J, Hoyle C, Roberts D
Department of Pathology, University of Liverpool, UK.
Histopathology. 1991 Nov;19(5):395-402. doi: 10.1111/j.1365-2559.1991.tb00228.x.
Using a mouse monoclonal antibody raised against recombinant human tumour necrosis factor alpha (TNF alpha), we have studied immunostained paraffin-embedded autopsy lung tissues from 13 patients dying with adult respiratory distress syndrome and 10 control patients dying of unrelated conditions. Material from 12 of the 13 cases showed positive staining, strong in six, less strong in the remaining six. TNF alpha was located principally within epithelial cells resembling type II pneumocytes in both early and late stages of adult respiratory distress syndrome. In the early stages the TNF alpha positive cells were scattered and appeared disorganized, but in later cases they formed an epithelium. The cells were confirmed as epithelial by their staining pattern with monoclonal antibodies reactive with epithelial, cytokeratin and macrophage antigens, and by double staining for epithelial membrane antigen and TNF alpha. In the control group, staining for TNF alpha was confined to very occasional plump type II pneumocytes, and a weak reaction in bronchial lining cells. Macrophages were only weakly stained in either group. It is suggested that TNF alpha is synthesized (or possibly absorbed) by type II pneumocytes and may be involved in the production of, or recovery from, adult respiratory distress syndrome.
我们使用一种针对重组人肿瘤坏死因子α（TNFα）产生的小鼠单克隆抗体，研究了13例死于成人呼吸窘迫综合征患者和10例死于无关疾病的对照患者经免疫染色的石蜡包埋尸检肺组织。13例中的12例材料显示阳性染色，6例强阳性，其余6例弱阳性。在成人呼吸窘迫综合征的早期和晚期，TNFα主要位于类似II型肺细胞的上皮细胞内。在早期，TNFα阳性细胞分散且排列紊乱，但在后期病例中它们形成了上皮。通过它们与上皮、细胞角蛋白和巨噬细胞抗原反应的单克隆抗体的染色模式，以及上皮膜抗原和TNFα的双重染色，证实这些细胞为上皮细胞。在对照组中，TNFα染色仅限于偶尔出现的丰满II型肺细胞，以及支气管内衬细胞中的弱阳性反应。两组中的巨噬细胞染色均较弱。提示TNFα由II型肺细胞合成（或可能被吸收），可能参与成人呼吸窘迫综合征的发生或恢复。
