Lukás F, Koppová I, Kudrna V, Kopecný J
Institute of Animal Physiology and Genetics, Academy of Science of the Czech Republic, 142 20 Prague, Czechia.
Folia Microbiol (Praha). 2007;52(1):99-104. doi: 10.1007/BF02932147.
Bacterial 16S rDNA from fecal samples of two calves were amplified by PCR and analyzed by denaturing gradient gel electrophoresis; selected bands were sequenced. Escherichia coli and Bifidobacterium animalis were the initial colonizers, followed by species closely related to the genera Bacteroides, Clostridium and Faecalibacterium. Change of diet was connected with shifts of bacterial population and with the occurrence of many bacterial species that have not been cultured up to now. The diet change corresponded with an alteration in a volatile-fatty-acid concentration in fecal samples.
通过聚合酶链反应（PCR）扩增两只小牛粪便样本中的细菌16S核糖体DNA（rDNA），并采用变性梯度凝胶电泳进行分析；对选定的条带进行测序。大肠杆菌和动物双歧杆菌是最初的定植菌，随后是与拟杆菌属、梭菌属和粪杆菌属密切相关的物种。饮食的改变与细菌种群的变化以及许多迄今尚未培养的细菌物种的出现有关。饮食变化与粪便样本中挥发性脂肪酸浓度的改变相对应。
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