Olvera A, Cerdà-Cuéllar M, Mentaberre G, Casas-Diaz E, Lavin S, Marco I, Aragon V
Centre de Recerca en Sanitat Animal (CReSA), Esfera UAB, Edifici CReSA, Campus de Bellaterra-UAB, 08193 Bellaterra, Barcelona, Spain.
Vet Microbiol. 2007 Nov 15;125(1-2):182-6. doi: 10.1016/j.vetmic.2007.05.003. Epub 2007 May 18.
Haemophilus parasuis is a colonizer of the upper respiratory tract of pigs and the etiological agent of Glässer's disease, which is characterized by a fibrinous polyserositis, meningitis and arthritis. Glässer's disease has never been reported in wild boar (Sus scrofa), although antibodies against H. parasuis have been detected. The goal of this study was to confirm the presence of this bacterium in wild boar by bacterial isolation and to compare the strains to H. parasuis from domesticated pigs. Therefore, nasal swabs from 42 hunted wild boars were processed for bacterial isolation and subsequent H. parasuis identification by specific PCR, biochemical tests and 16S rRNA gene sequencing. Two different strains of H. parasuis from two wild boars were isolated. These strains belonged to serotype 2 and were included by 16S rRNA gene sequencing and MLST analysis in a cluster with other H. parasuis strains of nasal origin from domestic pigs. During this study, Actinobacillus minor and Actinobacillus indolicus, which are NAD-dependent Pasteurellaceae closely related to H. parasuis, were also isolated. Our results indicate similarities in the respiratory microbiota of wild boars and domestic pigs, and although H. parasuis was isolated from wild boars, more studies are needed to determine if this could be a source of H. parasuis infection for domestic pigs.
副猪嗜血杆菌是猪上呼吸道的定植菌，也是格氏病的病原体，格氏病的特征为纤维素性多浆膜炎、脑膜炎和关节炎。尽管已检测到针对副猪嗜血杆菌的抗体，但格氏病从未在野猪（Sus scrofa）中报道过。本研究的目的是通过细菌分离来确认这种细菌在野猪中的存在，并将这些菌株与家猪的副猪嗜血杆菌进行比较。因此，对42头被猎杀野猪的鼻拭子进行处理，以进行细菌分离，并随后通过特异性PCR、生化试验和16S rRNA基因测序来鉴定副猪嗜血杆菌。从两头野猪中分离出了两种不同的副猪嗜血杆菌菌株。这些菌株属于2型血清型，通过16S rRNA基因测序和多位点序列分型分析，它们与来自家猪鼻腔来源的其他副猪嗜血杆菌菌株归为一个簇。在本研究过程中，还分离出了微小放线杆菌和产吲哚放线杆菌，它们是与副猪嗜血杆菌密切相关的依赖NAD的巴斯德菌科细菌。我们的结果表明野猪和家猪的呼吸道微生物群存在相似性，虽然从野猪中分离出了副猪嗜血杆菌，但还需要更多研究来确定这是否可能是家猪副猪嗜血杆菌感染的一个来源。
