Petersen Thomas, Niklason Laura
Department of Biomedical Engineering, 136 Hudson Hall, Duke University, Durham, NC 27708, USA.
Biomaterials. 2007 Sep;28(26):3751-6. doi: 10.1016/j.biomaterials.2007.05.012. Epub 2007 May 25.
Tissue engineering is a promising approach to aid in the treatment of a wide range of clinical disorders by developing replacement tissues for damaged or diseased organs. Such approaches, however, will require large and functional cell populations in order to produce a tissue that can replicate in vivo function. Most adult cells have limited replicative potential that limits their use in tissue engineering applications. Thus, cell populations with expanded lifespan or increased replicative potential are of interest. Stem cell-derived populations may allow the creation of large cell populations that have increased replicative potential over adult differentiated cells. In addition, ectopic human telomerase reverse transcriptase expression and induced B-cell lymphoma 2 (bcl-2) expression can allow adult cells to proliferate more extensively than unaltered cells. However, concerns for malignant transformation exist with telomerase and bcl-2 approaches. The current states of research in these areas are reviewed as they relate to tissue engineering and the cellular lifespan.
组织工程是一种很有前景的方法，通过为受损或患病器官开发替代组织来辅助治疗多种临床疾病。然而，此类方法需要大量且功能正常的细胞群体，以便产生能够在体内复制功能的组织。大多数成体细胞的复制潜力有限，这限制了它们在组织工程应用中的使用。因此，具有延长寿命或增加复制潜力的细胞群体备受关注。干细胞衍生的群体可能允许创建比成体分化细胞具有更高复制潜力的大量细胞群体。此外，异位人端粒酶逆转录酶表达和诱导的B细胞淋巴瘤2（bcl-2）表达可以使成体细胞比未改变的细胞更广泛地增殖。然而，端粒酶和bcl-2方法存在恶性转化的担忧。本文综述了这些领域与组织工程和细胞寿命相关的研究现状。
