Kindy M S, Brown K E, Sonenshein G E
Department of Biochemistry, University of Kentucky, Lexington 40536-0084.
J Cell Biochem. 1991 Aug;46(4):345-50. doi: 10.1002/jcb.240460409.
Vascular smooth muscle cells (SMCs) play a key role in the development of major arteries. Furthermore, abnormal growth of vascular smooth muscle cells has been implicated in the progression of major diseases of the cardiovascular system. Here, we report detection in primary cultures of bovine vascular smooth muscle cells of mRNA for two growth-state-related genes, 2F1 and 2A9, which code for a mitochondrial ADP/ATP carrier and calcyclin, respectively, and on the characterization of their cell cycle expression. Cultures of exponentially growing smooth muscle cells were made quiescent by serum deprivation. Upon readdition of serum, cells entered the cell cycle synchronously; DNA synthesis began 12 h post-serum addition. Levels of 2F1 and 2A9 RNA were low in quiescent cells and increased between 2 and 4 h post-serum addition. No changes in the rates of transcription of the 2F1 or 2A9 genes were detected by nuclear run-off assays during the time course. Thus the regulation of changes in expression of 2F1 and 2A9 in early G1 is mediated post-transcriptionally.
血管平滑肌细胞（SMCs）在大动脉发育中起关键作用。此外，血管平滑肌细胞的异常生长与心血管系统主要疾病的进展有关。在此，我们报告在原代培养的牛血管平滑肌细胞中检测到两个与生长状态相关基因2F1和2A9的mRNA，它们分别编码线粒体ADP/ATP载体和钙周期蛋白，并对其细胞周期表达进行了表征。通过血清剥夺使指数生长的平滑肌细胞培养物静止。重新添加血清后，细胞同步进入细胞周期；血清添加后12小时开始DNA合成。静止细胞中2F1和2A9 RNA水平较低，血清添加后2至4小时升高。在该时间进程中，通过核转录分析未检测到2F1或2A9基因转录速率的变化。因此，2F1和2A9在G1早期表达变化的调节是在转录后介导的。
