Zhang Zai-yun, Wu Jin-min
Department of Oncology, The Second Hospital of Shandong University, Jinan 250033, China.
Zhonghua Zhong Liu Za Zhi. 2007 Jan;29(1):17-20.
To explore the anti-tumor effects induced by fusion of interleukin (IL)-18 gene transfected lung cancer cell line NCI-H460 cells with dendritic cells (DC).
(1) DC were induced from human monocytes and fused with IL-18 transfected NCI-H460 cells. Fusion was selected using MACS microbeads. (2) Four groups (group GT, group PT, group NT and group BC) were set up. T cells activated by IL-18 gene transfected fusion or pcDNA3. 1 + vector transfected fusion and non-transfected fusion were taken as effetor cells. No effector cells was in group BC. Lactic dehydrogenase ( LDH) method was used to evaluate the antitumor effect in vitro. (3) Tumor-bearing nude mice were inoculated with effector cells mentioned above. The tumor size and weight in the 4 groups were compared.
The killing rate in vitro of 3 groups were 53. 14% ,30. 10% and 31.49% , respectively. The tumor size and weight in the 3 groups were lower than group BC, among which group GT was the lowest.
Fusion of IL-18 gene transfected NCI-H460 lung cancer cells with dendritic cells can effectively induce anti-tumor immunity in the host.
探讨白细胞介素（IL）-18基因转染的肺癌细胞系NCI-H460细胞与树突状细胞（DC）融合诱导的抗肿瘤作用。
（1）从人单核细胞诱导DC，并与IL-18转染的NCI-H460细胞融合。使用磁珠分选融合细胞。（2）设立四组（GT组、PT组、NT组和BC组）。将经IL-18基因转染融合体或pcDNA3.1+载体转染融合体及未转染融合体激活的T细胞作为效应细胞。BC组无效应细胞。采用乳酸脱氢酶（LDH）法体外评价抗肿瘤作用。（3）将上述效应细胞接种于荷瘤裸鼠。比较四组的肿瘤大小和重量。
三组的体外杀伤率分别为53.14%、30.10%和31.49%。三组的肿瘤大小和重量均低于BC组，其中GT组最低。
IL-18基因转染的NCI-H460肺癌细胞与树突状细胞融合可有效诱导宿主的抗肿瘤免疫。
