Din Jehangir N, Harding Scott A, Valerio Christopher J, Sarma Jaydeep, Lyall Karin, Riemersma Rudolph A, Newby David E, Flapan Andrew D
Centre for Cardiovascular Sciences, University of Edinburgh, Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, United Kingdom.
Atherosclerosis. 2008 Mar;197(1):290-6. doi: 10.1016/j.atherosclerosis.2007.04.047. Epub 2007 Jun 18.
Dietary intake of fish rich in omega-3 fatty acids is associated with a reduction in cardiovascular events. The mechanisms for this are uncertain and previous studies investigating effects on platelet function have produced inconsistent results. Platelet-monocyte aggregation is a sensitive marker of platelet activation and may contribute to the initiation and progression of atherothrombosis. This study assessed the effect of dietary intervention with oily fish on platelet-monocyte aggregation in healthy subjects.
Fourteen subjects had their diet supplemented with 500 g of oil-rich fish per week for 4 weeks. A control group of 14 subjects received no dietary intervention over a 4-week period. Platelet-monocyte aggregates were assessed with flow cytometry.
Dietary intervention with fish led to an increase in omega-3 fatty acids in plasma phospholipids (14.2+/-3.4% versus 5.8+/-1.3%, P<0.001). In contrast to the control group, platelet-monocyte aggregates were reduced by 35% following dietary intervention with oily fish (16.0+/-9.0% versus 24.8+/-10.9%, P<0.01), and returned to basal levels 4 weeks after discontinuation of supplementation. There was an inverse correlation between platelet-monocyte aggregation and plasma omega-3 fatty acid concentrations (r=-0.421, P=0.006). There were no changes in the plasma markers of platelet activation, soluble P-selectin or soluble CD40 ligand.
We have demonstrated, for the first time, that dietary intervention with oil-rich fish reduces platelet-monocyte aggregation in man. Our results suggest that reduced platelet activation provides a potential mechanism through which fish oils confer their cardiovascular preventative benefits.
摄入富含ω-3脂肪酸的鱼类与心血管事件减少有关。其机制尚不确定，以往研究对血小板功能影响的结果并不一致。血小板-单核细胞聚集是血小板活化的敏感标志物，可能有助于动脉粥样硬化血栓形成的起始和进展。本研究评估了食用油性鱼类的饮食干预对健康受试者血小板-单核细胞聚集的影响。
14名受试者每周饮食中补充500克富含油脂的鱼类，持续4周。14名受试者组成的对照组在4周内未接受饮食干预。采用流式细胞术评估血小板-单核细胞聚集物。
鱼类饮食干预导致血浆磷脂中ω-3脂肪酸增加（14.2±3.4%对5.8±1.3%，P<0.001）。与对照组相比，食用油性鱼类进行饮食干预后，血小板-单核细胞聚集物减少了35%（16.0±9.0%对24.8±10.9%，P<0.01），且在停止补充4周后恢复至基础水平。血小板-单核细胞聚集与血浆ω-3脂肪酸浓度呈负相关（r=-0.421，P=0.006）。血小板活化的血浆标志物可溶性P-选择素或可溶性CD40配体无变化。
我们首次证明，食用富含油脂的鱼类进行饮食干预可减少人体血小板-单核细胞聚集。我们的结果表明，血小板活化降低提供了一种潜在机制，通过该机制鱼油发挥其心血管预防益处。
