Hair Alan, Vassetzky Yegor
CNRS UMR 8126, Institut Gustave Roussy, Univ. Paris Sud XI, 94805 Villejuif Cedex, France.
J Cell Biochem. 2007 Dec 1;102(5):1140-8. doi: 10.1002/jcb.21413.
The size of the DNA loop containing the Xenopus laevis somatic 5S RNA gene cluster has been estimated using a simple, precise and sensitive method that we have developed for use on any tandemly arrayed DNA repeat region, and was found to increase during development We have found that after the mid-blastula transition, when transcription is activated in the embryo, a subset of somatic 5S RNA genes becomes specifically associated with the nuclear matrix. This association correlates with the transcriptional activity of the 5S genes.
我们已开发出一种简单、精确且灵敏的方法，可用于任何串联排列的DNA重复区域，借此估算出了包含非洲爪蟾体细胞5S RNA基因簇的DNA环的大小，结果发现其在发育过程中会增大。我们还发现，在囊胚中期转变之后，当胚胎中的转录被激活时，一部分体细胞5S RNA基因会与核基质特异性结合。这种结合与5S基因的转录活性相关。
