Guo Yi, Jiang Li, Yuan Ai-yun, Hu Yue, Li Xin
Department of Neurology, Chongqing University of Medical Sciences, Chongqing 400014, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007 Jun;19(6):343-5.
To explore the influence of duration of convulsion state (SC) on neuronal apoptosis, mitochondrial membrane potential (Deltapsim) and cytochrome C (cyt C) release in hippocampus in Wistar rats after SC.
SC lasting for 30 minutes or 3 hours was induced by intraperitoneal injection of lithium chloride and pilocarpine. Rats were sacrificed at 3, 6, 12 hours and on 1 day after 30 minutes SC and at 3, 6 hours and on 1 day after 3 hours SC. The apoptosis, mitochondrial Deltapsim and intracellular cyt C were determined with flow cytometry, and the correlation with SC duration was compared.
The proportion of apoptotic cells, the decrease in mitochondrial Deltapsim and the release of intracellular cyt C significantly changed at 30 minutes after SC. The peak level of apoptosis was seen at 12 th hour after SC and that of mitochondrial peaked at 6 th hour after SC in apoptosis and the two early apoptotic events, respectively. Compared with the same time point after 30 minutes SC, the levels of apoptosis and the two early apoptotic events after 3 hours SC were much higher. The neuronal apoptosis and the two early apoptotic events in hippocampus after SC showed positive correlation with the duration of SC in partial correlation analysis (all P<0.05).
Severe seizure could induce the changes in neuronal apoptosis and the early apoptotic events in hippocampus after SC. The longer the duration of SC is, the more serious change in apoptosis and early apoptotic events are.
探讨惊厥状态（SC）持续时间对Wistar大鼠惊厥后海马神经元凋亡、线粒体膜电位（ΔΨm）及细胞色素C（cyt C）释放的影响。
腹腔注射氯化锂和匹鲁卡品诱导持续30分钟或3小时的SC。在30分钟SC后的3、6、12小时及1天，以及3小时SC后的3、6小时及1天处死大鼠。采用流式细胞术检测凋亡、线粒体ΔΨm及细胞内cyt C，并比较其与SC持续时间的相关性。
SC后30分钟，凋亡细胞比例、线粒体ΔΨm降低及细胞内cyt C释放显著变化。凋亡时，凋亡峰值出现在SC后12小时，线粒体峰值出现在SC后6小时，分别为两个早期凋亡事件。与30分钟SC后的同一时间点相比，3小时SC后的凋亡水平及两个早期凋亡事件更高。部分相关性分析显示，SC后海马神经元凋亡及两个早期凋亡事件与SC持续时间呈正相关（均P<0.05）。
严重惊厥可诱导SC后海马神经元凋亡及早期凋亡事件发生变化。SC持续时间越长，凋亡及早期凋亡事件的变化越严重。
