Evans Amanda Lisabeth, Bryant James, Skepper Jeremy, Smith Stephen K, Print Cristin G, Charnock-Jones D Stephen
Department of Pathology, University of Cambridge, Tennis Court Rd, Cambridge, CB2 1QP, UK.
Angiogenesis. 2007;10(3):217-26. doi: 10.1007/s10456-007-9076-y. Epub 2007 Jun 19.
It has become increasingly clear that the investigation of vascular development is best considered in the context of a whole tissue environment since in vivo endothelial cells interact closely with other cell types. Murine embryoid bodies have been used as a model for the early development of a vascular network and are amenable to genetic manipulation and treatment with soluble modulators. However, quantifying morphological changes in these complex three-dimensional structures is challenging. In this paper we describe protocols to culture embryoid bodies on a large scale to study vascular development together with methods to quantify changes seen when antiangiogenic agents or endothelial cell-specific transgenes are introduced.
越来越明显的是，鉴于体内内皮细胞与其他细胞类型密切相互作用，血管发育的研究最好在整个组织环境的背景下进行。小鼠胚胎体已被用作血管网络早期发育的模型，并且适合进行基因操作和用可溶性调节剂处理。然而，量化这些复杂三维结构中的形态变化具有挑战性。在本文中，我们描述了大规模培养胚胎体以研究血管发育的方案，以及量化引入抗血管生成剂或内皮细胞特异性转基因时所观察到变化的方法。
