Starita-Geribaldi Mireille, Thebault Pascal, Taffin de Givenchy Elisabeth, Guittard Frederic, Geribaldi Serge
Connexines et Prolifération Germinale: Physiopathologie Cellulaire et Moléculaire (INSERM U670), UFR de Médecine, Université de Nice Sophia-Antipolis, Nice, France.
Electrophoresis. 2007 Jul;28(14):2489-97. doi: 10.1002/elps.200600598.
The synthesis of hemi-fluorinated zwitterionic surfactants was realized and assessed for 2-DE, a powerful separation method for proteomic analysis. These new fluorinated amidosulfobetaine (FASB-p,m) were compared to their hydrocarbon counterparts amidosulfobetaine (ASB-n) characterized by a hydrophilic polar head, a hydrophobic and lipophilic tail, and an amido group as connector. The tail of these FASB surfactants was in part fluorinated resulting in the modulation of its lipophilicity (or oleophobicity). Their effect on the red blood cell (RBC) membrane showed a specific solubilization depending on the length of the hydrophobic part. A large number of polypeptide spots appeared in the 2-DE patterns by using FASB-p,m. The oleophobic character of these surfactants was confirmed by the fact that Band 3, a highly hydrophobic transmembrane protein, was not solubilized by these fluorinated structures. The corresponding pellet was very rich in Band 3 and could then be solubilized by using a strong detergent such as amidosulfobetaine with an alkyl tail containing 14 carbon atoms (ASB-14). Thus, these hemi-fluorinated surfactants appeared as powerful tools when used at the first step of a two-step solubilization strategy using a hydrocarbon homologous surfactant in the second step.
实现了半氟化两性离子表面活性剂的合成，并对其用于二维电泳（2-DE，蛋白质组分析的一种强大分离方法）进行了评估。将这些新型氟化酰胺基磺基甜菜碱（FASB-p,m）与其碳氢化合物对应物酰胺基磺基甜菜碱（ASB-n）进行了比较，后者具有亲水性极性头、疏水性和亲脂性尾以及作为连接体的酰胺基。这些FASB表面活性剂的尾部部分氟化，从而调节了其亲脂性（或疏油性）。它们对红细胞（RBC）膜的作用显示出取决于疏水部分长度的特异性增溶作用。使用FASB-p,m时，二维电泳图谱中出现了大量多肽斑点。这些表面活性剂的疏油特性通过以下事实得到证实：带3，一种高度疏水的跨膜蛋白，不能被这些氟化结构增溶。相应的沉淀中富含带3，然后可以通过使用强去污剂如具有含14个碳原子的烷基尾的酰胺基磺基甜菜碱（ASB-14）来增溶。因此，当在两步增溶策略的第一步中使用这些半氟化表面活性剂，并在第二步中使用碳氢化合物同源表面活性剂时，它们表现为强大的工具。
