Britland S T, von Zimmermann O, Sharma A K, Bretzel R G, Federlin K
Dept. of Anatomy, University of Aberdeen, U.K.
J Neurol Sci. 1991 Oct;105(2):168-74. doi: 10.1016/0022-510x(91)90141-s.
Quantitative light and electronmicroscopical morphometric techniques were used to determine the effect of pancreatic islet transplantation on experimental diabetic neuropathy. Groups of STZ-diabetic rats were given islet transplants at 3 weeks after diabetes onset (prevention) and at 6 months after diabetes onset (reversal). Comparisons were made with onset controls, age-matched non-diabetic controls and untreated diabetic controls 6 months later (n = 8 for all groups). Euglycaemia and normal levels of glycosylated haemoglobin were achieved in both groups of diabetics after islet transplantation. Loss of body weight in diabetic animals was prevented by early islet transplantation, but was only partially reversed following delayed islet transplantation. Normal growth of myelinated fibres and axons during development was retarded in untreated diabetics, but was normal in rats given islet transplants soon after the onset of diabetes (cross-sectional perimeter and area). Diabetics transplanted with islets after a delay had myelinated fibres and axons with diminished calibre. Teased fibre preparations of nerves from diabetics which had received islet transplants showed no excess of abnormalities. This study has shown that the development of certain structural abnormalities of peripheral nerve fibres is prevented in diabetic rats which receive transplants of islets of Langerhans soon after the onset of diabetes. However, once established abnormal fibre morphology can not be completely ameliorated merely by achieving and sustaining euglycaemia through delayed islet transplantation.
采用定量光镜和电镜形态计量技术来确定胰岛移植对实验性糖尿病神经病变的影响。将链脲佐菌素诱导的糖尿病大鼠分为两组，一组在糖尿病发病后3周接受胰岛移植（预防组），另一组在糖尿病发病后6个月接受胰岛移植（逆转组）。6个月后，将这两组与发病对照组、年龄匹配的非糖尿病对照组和未治疗的糖尿病对照组进行比较（所有组n = 8）。胰岛移植后，两组糖尿病患者均实现了血糖正常和糖化血红蛋白水平正常。早期胰岛移植可防止糖尿病动物体重减轻，但延迟胰岛移植仅能部分逆转体重减轻。未治疗的糖尿病患者在发育过程中有髓纤维和轴突的正常生长受到阻碍，但糖尿病发病后不久接受胰岛移植的大鼠（横截面积和周长）则正常。延迟移植胰岛的糖尿病患者的有髓纤维和轴突直径减小。对接受胰岛移植的糖尿病患者的神经进行单纤维标本制备，未发现过多异常。本研究表明，糖尿病发病后不久接受胰岛移植 的糖尿病大鼠可预防周围神经纤维某些结构异常的发展。然而，一旦形成异常纤维形态，仅通过延迟胰岛移植实现并维持血糖正常，无法完全改善。
