Wang Hua-Zhong, Chen Ya-Ping, Chen Pei-Du
Chemistry and Life Science College, Tianjin Normal University, Tianjin 300074, China.
Sheng Wu Gong Cheng Xue Bao. 2007 May;23(3):367-74.
With the development of structural and functional genomics, nowadays specific plant genome and transcriptome sequences can be cloned much easier and faster. Next step is to identify the functions of different genes and regulating elements to unravel the genetic mechanisms behind plant growth and development. Expression and its regulation are the language and dynamic property of genetic material, so expression and regulation analysis of target genes and sequences in plant cell is the basis for function study. Besides stable genetic transformation, plant transient expression system gains broad application in recent years, and its combination with other new technologies as gene shuffling, VIGS and RNAi plays a more and more important role in plant functional genomics.
随着结构和功能基因组学的发展,如今特定植物的基因组和转录组序列克隆起来更加容易和快速。下一步是鉴定不同基因和调控元件的功能,以揭示植物生长发育背后的遗传机制。基因表达及其调控是遗传物质的语言和动态特性,因此植物细胞中靶基因和序列的表达与调控分析是功能研究的基础。除了稳定遗传转化外,植物瞬时表达系统近年来得到了广泛应用,并且它与基因改组、病毒诱导基因沉默(VIGS)和RNA干扰等其他新技术相结合,在植物功能基因组学中发挥着越来越重要的作用。