Meng Kewei, Song Zhanwen, Zhou Xianting
Division of Hepatobiliary-liver Transplantation Surgery, Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai, Shandong, 264000, P. R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2007 May;21(5):528-31.
To establish the rat orthotopic liver transplantation model by characterizing the blood supply of hepatic artery with the Cuff skill and the modified arterial sleeve anastomosis, to explore the possible mechanisms of acute rejection and the express of Fractalkine (Fkn) in the early stage after hepatic allograft operation.
SD rats were selected as donors and Wistar rats as receptor for the rejection model of orthotopic liver transplantation. Recipient rats were divided into 2 groups randomly after operation and the drugs were given intraperitoneally once a day in each group. In the experimental group, cyclosporine A (CsA) was delivered with 3 mg/kg. In the control group, only normal saline was given with 3 ml/kg. Condition of survivals were observed. The rejection activity index (RAI) and the expression of Fkn of liver tissue were observed after 3rd, 5th and 7th days in 5 rats. The rest of rats in each group were fed and given drug or normal saline until they were died and the mean survival time were recorded.
There were 18 survivals in control group, and 19 in experimental group after liver transplantation. Condition of survivals in experimental group was better than that of control group. The mean survival times of experimental group (19.50+/-4.51 days) was significantly longer than that of control group (7.60+/-1.60 days), showing statistically significant difference (P<0.05). After 3rd, 5th and 7th days of transplantation, RAI of control group were 3.80+/-0.35, 5.90+/-0.87 and 7.50+/-1.30, respectively; RAI of experimental group were 3.10+/-0.21, 3.90+/-0.41 and 4.50+/-0.52. There was statistically significant difference in RAI between 2 groups on the 7th day after transplantation (P<0.01). On the 3rd, 5th and 7th days after transplantation, the Fkn of control group was 8.20+/-0.57, 21.30+/-3.30 and 25.70+/-4.91, and that of experimental group was 8.30+/-0.56, 10.30+/-0.67 and 11.70+/- 1.23. There were statistically significant differences in Fkn between 2 groups on the 5th, 7th days after transplantation (P< 0.01).
Fkn is a participant in acute rejection after the rat orthotopic liver transplantation and can be chosen as a useful target in the diagnosis of acute rejection. CsA has immunosuppressive property in the condition of acute rejection in the rat orthotopic liver transplantation, which may be result from the decreased the level of Fkn.
采用套袖法及改良动脉袖套吻合法对肝动脉血供进行塑形，建立大鼠原位肝移植模型，探讨肝移植术后早期急性排斥反应的可能机制及趋化因子（Fkn）的表达。
选取SD大鼠作为供体，Wistar大鼠作为受体建立原位肝移植排斥反应模型。术后将受体大鼠随机分为2组，每组每天腹腔注射1次药物。实验组给予环孢素A（CsA）3mg/kg，对照组给予生理盐水3ml/kg。观察存活情况。于术后第3、5、7天观察5只大鼠的排斥反应活性指数（RAI）及肝组织Fkn表达。每组其余大鼠饲养并给予药物或生理盐水，直至死亡，记录平均存活时间。
肝移植后对照组存活18只，实验组存活19只。实验组存活情况优于对照组。实验组平均存活时间（19.50±4.51天）显著长于对照组（7.60±1.60天），差异有统计学意义（P<0.05）。移植后第3、5、7天，对照组RAI分别为3.80±0.35、5.90±0.87、7.50±1.30；实验组RAI分别为3.10±0.21、3.90±0.41、4.50±0.52。移植后第7天两组RAI差异有统计学意义（P<0.01）。移植后第3、5、7天，对照组Fkn分别为8.20±0.57、21.30±3.30、25.70±4.91，实验组Fkn分别为8.30±0.56、10.30±0.67、11.70±1.23。移植后第5、7天两组Fkn差异有统计学意义（P<0.01）。
Fkn参与大鼠原位肝移植术后急性排斥反应，可作为急性排斥反应诊断的有用靶点。CsA在大鼠原位肝移植急性排斥反应中具有免疫抑制作用，可能是由于Fkn水平降低所致。
